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PLGA/HAP 支架的合成与表征，该支架具有 DNA 功能化的磷酸钙纳米粒子，用于骨组织工程。

Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering.

机构信息

Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117, Essen, Germany.

Department of Chemical and Materials Engineering, Chang Gung University, Kweishan, Taoyuan, 333, Taiwan.

出版信息

J Mater Sci Mater Med. 2020 Nov 2;31(11):102. doi: 10.1007/s10856-020-06442-1.

DOI:10.1007/s10856-020-06442-1
PMID:33140175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7606283/
Abstract

Porous scaffolds of poly(lactide-co-glycolide) (PLGA; 85:15) and nano-hydroxyapatite (nHAP) were prepared by an emulsion-precipitation procedure from uniform PLGA-nHAP spheres (150-250 µm diameter). These spheres were then thermally sintered at 83 °C to porous scaffolds that can serve for bone tissue engineering or for bone substitution. The base materials PLGA and nHAP and the PLGA-nHAP scaffolds were extensively characterized by X-ray powder diffraction, infrared spectroscopy, thermogravimetry, differential scanning calorimetry, and scanning electron microscopy. The scaffold porosity was about 50 vol% as determined by relating mass and volume of the scaffolds, together with the computed density of the solid phase (PLGA-nHAP). The cultivation of HeLa cells demonstrated their high cytocompatibility. In combination with DNA-loaded calcium phosphate nanoparticles, they showed a good activity of gene transfection with enhanced green fluorescent protein (EGFP) as model protein. This is expected enhance bone growth around an implanted scaffold or inside a scaffold for tissue engineering.

摘要

聚（丙交酯-乙交酯）（PLGA; 85:15）和纳米羟基磷灰石（nHAP）的多孔支架通过从均匀的 PLGA-nHAP 球（150-250μm 直径）的乳液沉淀程序制备。然后将这些球在 83°C 下进行热烧结，制成可用于骨组织工程或骨替代的多孔支架。基础材料 PLGA 和 nHAP 以及 PLGA-nHAP 支架通过 X 射线粉末衍射、红外光谱、热重分析、差示扫描量热法和扫描电子显微镜进行了广泛的表征。通过与支架的质量和体积相关联，并结合固相（PLGA-nHAP）的计算密度，确定支架的孔隙率约为 50 体积%。用 HeLa 细胞培养表明其具有高细胞相容性。与负载 DNA 的磷酸钙纳米颗粒结合使用，它们作为模型蛋白显示出良好的基因转染活性，增强型绿色荧光蛋白（EGFP）。这有望增强植入支架周围或组织工程支架内的骨生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/12276ed42ef0/10856_2020_6442_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/3bff7e0bf1d9/10856_2020_6442_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/7078998c2249/10856_2020_6442_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/9934e20d78c0/10856_2020_6442_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/953b2319c66a/10856_2020_6442_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/7eb7c03136ad/10856_2020_6442_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/9dff26952a94/10856_2020_6442_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/84c39874b7a6/10856_2020_6442_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/ab1bd7f4de0f/10856_2020_6442_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/88db571b9689/10856_2020_6442_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/7985eabd8a6f/10856_2020_6442_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/592b4262b644/10856_2020_6442_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/4e17a58a7a89/10856_2020_6442_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/12276ed42ef0/10856_2020_6442_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/3bff7e0bf1d9/10856_2020_6442_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/7078998c2249/10856_2020_6442_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/9934e20d78c0/10856_2020_6442_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/953b2319c66a/10856_2020_6442_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/7eb7c03136ad/10856_2020_6442_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/9dff26952a94/10856_2020_6442_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/84c39874b7a6/10856_2020_6442_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/ab1bd7f4de0f/10856_2020_6442_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/88db571b9689/10856_2020_6442_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/7985eabd8a6f/10856_2020_6442_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/592b4262b644/10856_2020_6442_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/4e17a58a7a89/10856_2020_6442_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5214/7606283/12276ed42ef0/10856_2020_6442_Fig13_HTML.jpg

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本文引用的文献

[1]
Well-ordered biphasic calcium phosphate-alginate scaffolds fabricated by multi-channel 3D plotting under mild conditions.

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[2]
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J Mater Sci Mater Med. 2019-1-22

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Dental pulp stem cells in chitosan/gelatin scaffolds for enhanced orofacial bone regeneration.

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Acta Biomater. 2018-11-29

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