Shi Ming, Xuan Liuyang, Zhang Yiling, Wang Dandan, Ye Feng, Shi Xuetao, Li Yan
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, Guangdong, PR China; Guangdong Provincial Engineering and Technology Center of Advanced and Portable Medical Devices, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing PR China.
Colloids Surf B Biointerfaces. 2019 Nov 1;183:110437. doi: 10.1016/j.colsurfb.2019.110437. Epub 2019 Aug 12.
Limited dimensional stability and osteogenic induction property of poly (lactide-co-glycolide) (PLGA) electrospun membranes hampered their applications in bone tissue engineering. Thermal treatment of fixed PLGA membranes at 50 °C for 2 h and further immersion in 75% ethanol at free-standing state were adopted in order to obtain a high stability and well-maintained fiber morphology. After the process, free-standing membranes were stable during incubation in PBS at 37 °C, the volumetric ratio was 59.0%, fibers became curved, and the average diameter was 816 nm. For as-electrospun PLGA membranes, the volumetric ratio was only 35.3%, showing that thermal treatment was effective to improve the dimensional stability. The addition of calcium phosphate nanoparticles in P5-T-F further increased the volumetric ratio (64.0%) and significantly improved the mechanical properties. The mineralization capacity of PLGA membranes was enhanced because of thermal treatment. Hemolysis ratios of all samples were ∼2% indicating good hemocompatibility of PLGA electrospun membranes. Proliferation of adipose derived stem cells from rats (rADSCs) on treated PLGA membranes was significantly faster than that on untreated one, especially for sample P-T-F. In addition to thermal treatment, the addition of calcium phosphate nanoparticles showed synergistic effects on improving mineralization property and osteogenic differentiation of rADSCs. When compared with P-T-F, P5-T-F had 153.0% higher ALP activity and 518% higher calcium mineral deposition based on alizarin red assay. Thermal treatment along with encapsulation of calcium phosphate nanoparticles in PLGA electrospun membranes demonstrated a great prospect for applications in bone tissue engineering.
聚乳酸-羟基乙酸共聚物（PLGA）电纺膜的尺寸稳定性有限和成骨诱导性能阻碍了它们在骨组织工程中的应用。为了获得高稳定性和良好维持的纤维形态，对固定的PLGA膜在50°C下热处理2小时，并在自由状态下进一步浸入75%乙醇中。处理后，自由状态的膜在37°C的PBS中孵育期间稳定，体积比为59.0%，纤维变弯曲，平均直径为816nm。对于静电纺丝的PLGA膜，体积比仅为35.3%，表明热处理有效地提高了尺寸稳定性。在P5-T-F中添加磷酸钙纳米颗粒进一步提高了体积比（64.0%）并显著改善了力学性能。由于热处理，PLGA膜的矿化能力增强。所有样品的溶血率约为2%，表明PLGA电纺膜具有良好的血液相容性。大鼠脂肪来源干细胞（rADSCs）在处理过的PLGA膜上的增殖明显快于未处理的膜，特别是对于样品P-T-F。除了热处理外，添加磷酸钙纳米颗粒对改善rADSCs的矿化性能和成骨分化具有协同作用。与P-T-F相比，基于茜素红测定，P5-T-F的碱性磷酸酶活性高153.0%，钙矿沉积高518%。热处理以及在PLGA电纺膜中封装磷酸钙纳米颗粒在骨组织工程中的应用显示出巨大的前景。
