Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80/82, CCB-Center for Chemistry and Biomedicine, 6020, Innsbruck, Austria.
Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80/82, CCB-Center for Chemistry and Biomedicine, 6020, Innsbruck, Austria.
J Pharm Biomed Anal. 2021 Jan 30;193:113686. doi: 10.1016/j.jpba.2020.113686. Epub 2020 Oct 22.
Near-infrared (NIR) spectroscopy is a powerful tool for qualitative and quantitative phytoanalysis. It is a rapid and high-throughput analytical method, with on-site capability, high chemical specificity, and no/minimal sample preparation. NIR spectroscopy is a powerful non-invasive and low-cost alternative with significant practical advantages compared to the conventional methods of analysis. These advantages are particularly exposed in the field of phytoanalysis. In contrast to synthetic medicines, natural products feature chemical diversity that can vary depending on the medicinal plant cultivation conditions, geographical origin or harvest time. The content of bioactive compounds and their derivatives, and thus, the quality parameters of the natural medicine need to be controlled with respect to a number of conditions. NIR spectroscopy has been proved to be particularly competitive in such difficult scenarios. In recent years, remarkable advances in the field of spectroscopic instrumentation and methods of analysis have appeared. Noteworthy was the appearance and dynamic continuing development of miniaturized, on-site capable NIR spectrometers. This was accompanied by application of new tools increasing the potential and reliability of NIR spectroscopy in phytoanalytical applications. The present review discussed the major principles of this technique and critically assesses its future application potential in phytoanalytical strategies. Major attention is given to the current development trends based on the most recent literature published in the field.
近红外（NIR）光谱学是定性和定量植物分析的有力工具。它是一种快速、高通量的分析方法，具有现场分析能力、高化学特异性和最小/无需样品制备。与传统分析方法相比，NIR 光谱学是一种强大的非侵入性和低成本替代方法，具有显著的实际优势。这些优势在植物分析领域尤为明显。与合成药物不同，天然产物的化学多样性可能因药用植物的栽培条件、地理来源或收获时间而异。因此，需要控制生物活性化合物及其衍生物的含量，以及天然药物的质量参数。NIR 光谱学已被证明在这种困难的情况下具有特别的竞争力。近年来，光谱仪器和分析方法领域出现了显著的进展。值得注意的是，小型化、现场分析能力的 NIR 光谱仪的出现和动态持续发展。与此同时，新工具的应用增加了 NIR 光谱学在植物分析应用中的潜力和可靠性。本文综述了该技术的主要原理，并批判性地评估了其在植物分析策略中的未来应用潜力。主要关注基于该领域最新文献的当前发展趋势。
