Faculty of Science, Department of Chemistry, Hacettepe University, Ankara, Turkey.
Advanced Technologies Application and Research Center, Hacettepe University, Ankara, Turkey.
Colloids Surf B Biointerfaces. 2021 Jan;197:111435. doi: 10.1016/j.colsurfb.2020.111435. Epub 2020 Oct 25.
In this study, we introduced a new strategy to design interface imprinted polymers for a novel aspect of molecular imprinting technique, utilization of sacrificial metal oxide particles. In the first step, bovine hemoglobin (BHb) was adsorbed on zinc oxide (ZnO) particles, which were then used to synthesize polyacrylic acid-based molecular imprinting membrane by bulk polymerization in the presence of ethylene glycol dimethacrylate as a cross-linking agent. After polymerization terminated, BHb-ZnO particles were removed to leave effective imprint sites onto the bulk polymeric network which is responsible for the formation of template orientation. The characterization of membranes was investigated by using Fourier transform infrared (FTIR), Raman spectroscopy (RS), scanning electron microscopy (SEM), surface area measurements (BET analyses) and thermogravimetric analysis (TGA). The interface molecularly imprinted membranes (iMIMs) have a relatively high specific rebinding capacity of 65.98 mg/g and excellent selectivity towards BHb with a separation factor of 6.78. The equilibrium adsorption isotherms fitted well to Langmuir isotherms (R = 0.9944) and the value of adsorption capability (Q) and equilibrium constant (b) were estimated to be 73.53 mg/g and 1.36 mg/mL for the iMIM, respectively. The kinetics of adsorption fitted best to pseudo-second order (R = 0.9912). The ZnO particles were used not only to ensure the preservation of the imprint cavities in the polymer network but also to lead to high template removal and better rebinding kinetics. This novel design with multiple recognition sites is quite simple and suitable for the separation of biomacromolecules.
在这项研究中，我们引入了一种新的策略来设计界面印迹聚合物，这是分子印迹技术的一个新方面，利用牺牲型金属氧化物颗粒。在第一步中，牛血红蛋白 (BHb) 被吸附在氧化锌 (ZnO) 颗粒上，然后在乙二醇二甲基丙烯酸酯作为交联剂的存在下，通过本体聚合合成基于聚丙烯酸的分子印迹膜。聚合终止后，去除 BHb-ZnO 颗粒，在本体聚合网络上留下有效的印迹位点，该位点负责模板取向的形成。通过傅里叶变换红外（FTIR）、拉曼光谱（RS）、扫描电子显微镜（SEM）、表面积测量（BET 分析）和热重分析（TGA）对膜进行了表征。界面分子印迹膜（iMIM）具有相对较高的特异性结合能力（65.98mg/g）和对 BHb 的优异选择性（分离因子为 6.78）。平衡吸附等温线很好地符合朗缪尔等温线（R=0.9944），吸附能力（Q）和平衡常数（b）的值分别为 73.53mg/g 和 1.36mg/mL。吸附动力学最好符合拟二级动力学（R=0.9912）。ZnO 颗粒不仅确保了印迹空穴在聚合物网络中的保存，而且还导致模板去除率高和更好的结合动力学。这种具有多个识别位点的新颖设计非常简单，适用于生物大分子的分离。
