Liu Ziwei, Wang Yuzhi, Xu Fangting, Wei Xiaoxiao, Chen Jing, Li Heqiong, He Xiyan, Zhou Yigang
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Anal Chim Acta. 2020 Sep 8;1129:49-59. doi: 10.1016/j.aca.2020.06.052. Epub 2020 Jul 11.
A novel magnetic molecularly imprinted polymer only based on deep eutectic solvents (DESs-MMIP) was successfully synthesized. The DESs-MMIP was constructed by using 2-hydroxyethyl methacrylate/tetrabutylammonium chloride deep eutectic solvent (DES) as functional monomer, arylamide/(3-acrylamidopropyl) trimethylammonium chloride deep eutectic solvent (DES) as cross-linker and bovine hemoglobin (BHb) as template through surface imprinting technology. The obtained DESs-MMIP was characterized by transmission electron microscope, X-ray diffraction, fourier transform infrared spectrometry, thermal gravimetric analysis and vibrating sample magnetometer. Under the optimized conditions, the maximum adsorption capacity of DESs-MMIP on BHb was 229.54 mg g and the imprinting factor reached up to 21.89. The selective adsorption experiments indicated that compared with seven references, DESs-MMIP showed significant selectivity for BHb. The new-type DESs-MMIP exhibited higher adsorption capacity and imprinting factor on BHb than molecularly imprinted polymers constructed with traditional functional monomer and cross-linker in reported methods. The recognition of BHb by DESs-MMIP in calf blood samples demonstrated the practicality of the particles. The DESs-MMIP only based on deep eutectic solvents with excellent selectivity is expected to become an ideal candidate for selective recognition of BHb in complicated samples.
成功合成了一种仅基于低共熔溶剂的新型磁性分子印迹聚合物（DESs-MMIP）。该DESs-MMIP通过表面印迹技术构建，以甲基丙烯酸2-羟乙酯/四丁基氯化铵低共熔溶剂（DES）为功能单体，芳酰胺/（3-丙烯酰胺基丙基）三甲基氯化铵低共熔溶剂（DES）为交联剂，牛血红蛋白（BHb）为模板。通过透射电子显微镜、X射线衍射、傅里叶变换红外光谱、热重分析和振动样品磁强计对所得的DESs-MMIP进行了表征。在优化条件下，DESs-MMIP对BHb的最大吸附容量为229.54 mg/g，印迹因子高达21.89。选择性吸附实验表明，与七种参比物相比，DESs-MMIP对BHb表现出显著的选择性。新型DESs-MMIP对BHb的吸附容量和印迹因子高于报道方法中用传统功能单体和交联剂构建的分子印迹聚合物。DESs-MMIP对小牛血样中BHb的识别证明了该颗粒的实用性。仅基于低共熔溶剂且具有优异选择性的DESs-MMIP有望成为复杂样品中选择性识别BHb的理想候选物。
