State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, PR China.
State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, PR China.
Carbohydr Polym. 2021 Jan 1;251:117044. doi: 10.1016/j.carbpol.2020.117044. Epub 2020 Sep 6.
Seed mucilage has significant economic value. However, the identification of key regulatory genes in mucilage formation and their molecular regulatory mechanism remain unknown. Artemisia sphaerocephala seeds are rich in mucilage. In this study, A. sphaerocephala seeds in 10, 20, 30, 40, 50, 60 and 70 days after flowering were used as materials to reveal their molecular regulatory mechanism in mucilage formation by RNA-sequencing and weighted gene co-expression network analysis (WGCNA). 21 key regulatory genes for mucilage formation were identified, including AsKNAT7 and AsTTG1 genes, as well as AsNAM and AsAP2 gene families. From 10-30 days after flowering, both AsNAM and AsAP2 supported mucilage formation. From 40-70 days after flowering, promotion by AsNAM and AsAP2 was weakened and the up-regulation of AsKNAT7 inhibited mucilage formation, leading to no further increases in mucilage content. This in depth elucidation of seed mucilage formation lays the foundation for the application of mucilage.
种皮黏液具有重要的经济价值。然而，种皮黏液形成过程中的关键调控基因及其分子调控机制仍不清楚。苍耳种子富含黏液。本研究以开花后 10、20、30、40、50、60 和 70 天的苍耳种子为材料，通过 RNA 测序和加权基因共表达网络分析（WGCNA）揭示了其在黏液形成过程中的分子调控机制。鉴定出 21 个与黏液形成相关的关键调控基因，包括 AsKNAT7 和 AsTTG1 基因以及 AsNAM 和 AsAP2 基因家族。开花后 10-30 天，AsNAM 和 AsAP2 均支持黏液形成。开花后 40-70 天，AsNAM 和 AsAP2 的促进作用减弱，AsKNAT7 的上调抑制了黏液形成，导致黏液含量不再增加。本研究深入阐明了种子黏液的形成机制，为黏液的应用奠定了基础。
