载有乳链菌肽的纳米晶细菌纤维素对选定肉品腐败乳酸菌的抗菌效果。
Antimicrobial efficacy of nisin-loaded bacterial cellulose nanocrystals against selected meat spoilage lactic acid bacteria.
机构信息
School of Public Health, Faculty of Health Sciences, Curtin University, Bentley, Western Australia, 6102, Australia.
Biotechnology and Drug Development Research Laboratory, Curtin Health Innovation Research Institute, School of Pharmacy and Biomedical Sciences, Curtin University, Bentley, Western Australia, 6102, Australia.
出版信息
Carbohydr Polym. 2021 Jan 1;251:117096. doi: 10.1016/j.carbpol.2020.117096. Epub 2020 Sep 16.
This study aimed to explore the potential of bacterial cellulose nanocrystals (BCNs) loaded with nisin against selected meat spoilage lactic acid bacteria (LAB) in vitro. BCNs were produced by HSO hydrolysis, and nisin-loaded BCNs were produced through the complexation method. All nanocrystals were assessed for their zeta-potential, encapsulation efficiency and antimicrobial activity. Different nisin concentrations were tested and the most effective nanocrystals were further characterised. BCNs had an average zeta-potential of - 43 mV and all nisin-loaded BCNs produced with 5 mg/ml BCNs suspension had zeta-potential values ≥- 30 mV. The encapsulation efficiency of nisin varied from 80.5 to 93.3 % and crystallinity of BCNs was not influenced by nisin encapsulation. Microbial inactivation was achieved by BCN loaded with 2.0 and 2.5 mg/ml nisin. Therefore, nisin-loaded BCNs may be used as antimicrobial agents in active food packaging.
本研究旨在探索负载乳链菌肽（nisin）的细菌纤维素纳米晶体（BCNs）在体外对选定的肉类腐败乳酸菌（LAB）的潜在应用。BCNs 通过 HSO 水解制备，通过复合物制备负载 nisin 的 BCNs。所有纳米晶体均评估了其 ζ 电位、包封效率和抗菌活性。测试了不同的 nisin 浓度，并进一步对最有效的纳米晶体进行了表征。BCNs 的平均 ζ 电位为-43mV，所有用 5mg/ml BCNs 悬浮液制备的负载 nisin 的 BCNs 的 ζ 电位值均≥-30mV。nisin 的包封效率在 80.5%至 93.3%之间变化，而 nisin 的包封并不影响 BCNs 的结晶度。BCN 负载 2.0 和 2.5mg/ml nisin 可实现微生物失活。因此，负载 nisin 的 BCNs 可用作活性食品包装中的抗菌剂。
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