Hu Yao, Xing Kequan, Li Xiaojing, Sang Shangyuan, McClements David Julian, Chen Long, Long Jie, Jiao Aiquan, Xu Xueming, Wang Jinpeng, Jin Zhengyu, Qiu Chao
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, 214122, China.
College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
NPJ Sci Food. 2023 May 20;7(1):20. doi: 10.1038/s41538-023-00181-7.
Nisin is a natural bacteriocin that exhibits good antibacterial activity against Gram-positive bacteria. It has good solubility, stability, and activity under acidic conditions, but it becomes less soluble, stable, and active when the solution pH exceeds 6.0, which severely restricted the industrial application range of nisin as antibacterial agent. In this study, we investigated the potential of complexing nisin with a cyclodextrin carboxylate, succinic acid-β-cyclodextrin (SACD), to overcome the disadvantages. Strong hydrogen bonding was shown between the nisin and SACD, promoting the formation of nisin-SACD complexes. These complexes exhibited good solubility under neutral and alkaline conditions, and good stability after being held at high pH values during processing with high-steam sterilization. Moreover, the nisin-SACD complexes displayed significantly improved antibacterial activity against model Gram-positive bacteria (S. aureus). This study shows that complexation can improve the efficacy of nisin under neutral and alkaline situations, which may greatly broaden its application range in food, medical, and other industries.
乳酸链球菌素是一种天然的细菌素，对革兰氏阳性菌具有良好的抗菌活性。它在酸性条件下具有良好的溶解性、稳定性和活性，但当溶液pH值超过6.0时，其溶解性、稳定性和活性会降低，这严重限制了乳酸链球菌素作为抗菌剂的工业应用范围。在本研究中，我们研究了将乳酸链球菌素与环糊精羧酸盐琥珀酸-β-环糊精（SACD）复合以克服这些缺点的可能性。结果表明，乳酸链球菌素与SACD之间存在强氢键，促进了乳酸链球菌素-SACD复合物的形成。这些复合物在中性和碱性条件下具有良好的溶解性，并且在高温蒸汽灭菌处理过程中在高pH值下保持后具有良好的稳定性。此外，乳酸链球菌素-SACD复合物对模型革兰氏阳性菌（金黄色葡萄球菌）显示出显著提高的抗菌活性。本研究表明，复合作用可以提高乳酸链球菌素在中性和碱性条件下的功效，这可能会大大拓宽其在食品、医疗和其他行业的应用范围。
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