Pulmuone Co., Ltd., Cheongju 28164, Korea.
Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
Molecules. 2020 Oct 30;25(21):5056. doi: 10.3390/molecules25215056.
PMO 08 was evaluated as a starter culture for plant-based probiotic beverages. Its viability under various culture conditions and acidification ability in standardized tomato medium, fermentation parameters, and beverage properties were assessed. PMO 08 could grow under various culture conditions; there was a high correlation between the incubation time to reach the optimal conditions and the inoculation concentration of lactic acid bacteria (LAB) (r = 0.997). Acidity (0.958 ± 0.002%) and LAB count (9.78 ± 0.14 Log CFU/mL) were significantly higher when fermented with than with the yogurt starter culture. A survival rate of 96% and 95% in artificial gastric juice and artificial intestinal juice, respectively, indicated that the probiotic requirements were met. The total polyphenol and glutamine content, and antioxidant activity increased after fermentation. The proline content significantly increased in PMO 08- fermented beverage. Thus PMO 08 is an effective starter culture for non-dairy probiotic beverages whose functional quality may be improved by fermentation.
PMO 08 被评估为植物性益生菌饮料的起始培养物。评估了其在各种培养条件下的生存能力和在标准化番茄培养基中的酸化能力、发酵参数和饮料特性。PMO 08 可以在各种培养条件下生长；达到最佳条件的培养时间与乳酸菌（LAB）接种浓度之间存在高度相关性（r = 0.997）。与酸奶起始培养物相比，用 发酵时酸度（0.958 ± 0.002%）和 LAB 计数（9.78 ± 0.14 Log CFU/mL）显著更高。在人工胃液和人工肠液中的存活率分别为 96%和 95%，表明符合益生菌要求。发酵后总多酚和谷氨酰胺含量以及抗氧化活性增加。脯氨酸含量在 PMO 08-发酵饮料中显著增加。因此，PMO 08 是一种有效的非乳制品益生菌饮料起始培养物，其功能质量可以通过发酵得到改善。
