Ishimatsu T, Nakayama I
First Department of Pathology, Medical College of Oita, Japan.
Acta Pathol Jpn. 1987 Aug;37(8):1207-21. doi: 10.1111/j.1440-1827.1987.tb00455.x.
Localization of ferritin using a pre-embedding diffusion technique and an indirect localization sequence has been made in 34 cases of human liver under normal and several pathological conditions. With light microscopic observation, positive immuno-staining for ferritin was demonstrated as diffuse deposits in the hepatocytes and Kupffer cells. Intensity of the positive immuno-staining for ferritin in these cells appeared to roughly coincide with serum ferritin levels of each patient, but showed no disease specificity, although hepatoma cells contained weak deposits or were negative from immuno-staining for ferritin. With electron microscopic studies, intracellular antigen was well preserved in the hepatocytes and Kupffer cells in most cases with the positive immuno-staining for ferritin being observed in cytosol and a few cisternae of rough endoplasmic reticulum. Content of the positive immuno-staining for ferritin differed considerably from one case to another and one cell to another even in the same case. There was no immuno-staining for ferritin in hemosiderin pigment, lysosome, most of rough endoplasmic reticulum, Golgi complexes, and nucleus in both cells.
运用包埋前扩散技术和间接定位序列对34例正常及多种病理状态下的人体肝脏进行铁蛋白定位。通过光学显微镜观察，铁蛋白的阳性免疫染色表现为肝细胞和库普弗细胞中的弥漫性沉积物。这些细胞中铁蛋白阳性免疫染色的强度似乎与每位患者的血清铁蛋白水平大致相符，但未显示出疾病特异性，尽管肝癌细胞中铁蛋白沉积物较弱或免疫染色呈阴性。通过电子显微镜研究，在大多数铁蛋白阳性免疫染色的病例中，肝细胞和库普弗细胞内的抗原保存良好，在细胞质和一些粗面内质网池内可观察到铁蛋白阳性免疫染色。即使在同一病例中，铁蛋白阳性免疫染色的含量在不同病例和不同细胞之间也有很大差异。在含铁血黄素色素、溶酶体、大部分粗面内质网、高尔基体复合体和细胞核中，两种细胞均未检测到铁蛋白免疫染色。
