Nakayama I, Noguchi S, Yamashita H, Murakami N, Mochizuki Y, Yokoyama S, Noguchi A, Mifune K
Acta Pathol Jpn. 1985 May;35(3):591-603. doi: 10.1111/j.1440-1827.1985.tb00601.x.
Intracellular localization of thyroglobulin (TG) using a pre-embedding diffusion technique and an indirect localization sequence has been made in human thyroid obtained from 20 patients with treated Grave's disease. Both antibodies, anti-human TG-rabbit IgG F(ab')2 and anti-rabbit IgG F(ab')2-goat IgG F(ab')2 fragments easily penetrated the cytoplasm of follicular cells which were dissociated by RPMI-1640 solution containing collagenase, dispase, and deoxyribonuclease. With light microscopic observation of semithin sections positive immuno-reaction for TG was demonstrated as fine granular deposits in the cytoplasm of the dissociated cells. In electron microscopic studies, intracellular antigen was well circumscribed within certain cell organelles in all cases with the positive immuno-reaction for TG being observed in perinuclear space, rough endoplasmic reticulum, Golgi complexes, secretory granules, and reabsorbed colloid droplets. Content of positive immuno-reaction product differed somewhat from one case to another and from one follicle to another even in the same case. There was no immunoreaction product in nuclei, mitochondria, lysosomes, and lipofuscin-like granules.
利用包埋前扩散技术和间接定位序列，对20例接受过治疗的格雷夫斯病患者的人甲状腺组织中的甲状腺球蛋白（TG）进行了细胞内定位研究。抗人TG兔IgG F(ab')2和抗兔IgG F(ab')2 - 山羊IgG F(ab')2片段这两种抗体都能轻易穿透由含有胶原酶、分散酶和脱氧核糖核酸酶的RPMI - 1640溶液解离的滤泡细胞的细胞质。在半薄切片的光学显微镜观察中，TG的阳性免疫反应表现为解离细胞细胞质中的细颗粒状沉积物。在电子显微镜研究中，在所有病例中，细胞内抗原都被明确限定在某些细胞器内，在核周间隙、粗面内质网、高尔基体复合体、分泌颗粒和再吸收的胶体小滴中观察到TG的阳性免疫反应。即使在同一病例中，阳性免疫反应产物的含量在不同病例之间以及不同滤泡之间也略有差异。在细胞核、线粒体、溶酶体和脂褐素样颗粒中没有免疫反应产物。
