Okada K, Nakayama I, Kobayashi M
Acta Pathol Jpn. 1987 Jun;37(6):915-28. doi: 10.1111/j.1440-1827.1987.tb00441.x.
Intra and extracellular localization of alpha-fetoprotein (AFP) has been studied by an indirect peroxidase labeled antibody method using 12 cases of human hepatocellular carcinoma (HCC). With light microscopic observation, positive immuno-staining for AFP was observed in 6 out of 12 cases and demonstrated as granular or diffuse deposits in the cytoplasm of neoplastic hepatocytes. In electron microscopic studies, 8 cases showed the positive immuno-staining for AFP in the neoplastic hepatocytes. Intracellular antigen was well circumscribed within certain cell organelles with the positive immuno-staining for AFP being observed in perinuclear space, cisternae of rough endoplasmic reticulum (RER), Golgi complexes, and secretory vesicles. In addition, the positive immuno-staining for AFP was observed in bile canaliculus-like space in most cases with increased levels serum AFP and in some cases which showed normal levels of serum AFP. Furthermore, the positive immuno-staining for AFP was also observed in intercellular, Disse's-like and sinusoid-like spaces, and micropinocytotic and lysosome-like vesicles in the endothelial cells in a few cases which showed excessively high value of serum AFP.
采用间接过氧化物酶标记抗体法，对12例人肝细胞癌（HCC）进行了甲胎蛋白（AFP）的细胞内与细胞外定位研究。通过光学显微镜观察，12例中有6例观察到AFP免疫染色呈阳性，表现为肿瘤性肝细胞胞质内的颗粒状或弥漫性沉积。在电子显微镜研究中，8例肿瘤性肝细胞中观察到AFP免疫染色呈阳性。细胞内抗原在某些细胞器内界限分明，在核周间隙、粗面内质网池、高尔基体复合体和分泌小泡中观察到AFP免疫染色呈阳性。此外，多数血清AFP水平升高的病例以及部分血清AFP水平正常的病例，在胆小管样间隙中观察到AFP免疫染色呈阳性。此外，少数血清AFP值过高的病例，在内皮细胞的细胞间、狄氏间隙样和窦状隙样间隙以及微饮小泡和溶酶体样小泡中也观察到AFP免疫染色呈阳性。
