Toyofuku H, Hayashi K, Awai M
Department of Pathology, Okayama University Medical School, Japan.
Acta Pathol Jpn. 1987 Aug;37(8):1279-90. doi: 10.1111/j.1440-1827.1987.tb00460.x.
The experiment was focused on clarifying changes in fibrin or fibrinogen related materials (FRMs) in blood, urine, and renal tissues of rats with disseminated intravascular coagulation (DIC). DIC was induced by a continuous infusion of massive volume of physiologic saline (100 ml) immediately after endotoxin injection. FRM response was checked by biochemical and histochemical examinations at various intervals. In the blood of DIC rats, platelet and fibrinogen levels initially decreased, followed by an increasing plasma fibrin degradation products (FDP). Parallel with elevation of blood FDP the percentage of glomeruli with FRMs increased. Thereafter, FRMs were observed in renal tubuli and urine. Our observations indicated that FRMs in renal tubuli were derived from glomerular capillaries via Bowman's space. In conclusion, in DIC the immunoenzymehistochemical (IEH) procedure appeared necessary for an accurate pathological diagnosis, and the presence of FRMs in renal tubuli appeared to be an important finding even in absence of FRMs in glomeruli.
该实验着重阐明弥散性血管内凝血（DIC）大鼠血液、尿液及肾组织中纤维蛋白或纤维蛋白原相关物质（FRMs）的变化。在内毒素注射后立即持续输注大量生理盐水（100 ml）诱导DIC。在不同时间间隔通过生化和组织化学检查来检测FRM反应。在DIC大鼠血液中，血小板和纤维蛋白原水平最初下降，随后血浆纤维蛋白降解产物（FDP）增加。随着血液FDP升高，有FRMs的肾小球百分比增加。此后，在肾小管和尿液中观察到FRMs。我们的观察表明，肾小管中的FRMs是通过鲍曼间隙从肾小球毛细血管衍生而来。总之，在DIC中，免疫酶组织化学（IEH）程序似乎是准确病理诊断所必需的，即使肾小球中没有FRMs，肾小管中存在FRMs似乎也是一个重要发现。
