TMA 技术与常规全切片分析在评估喉鳞状细胞癌增殖标志物表达中的比较。
Comparison of TMA Technique and Routine Whole Slide Analysis in Evaluation of Proliferative Markers Expression in Laryngeal Squamous Cell Cancer.
机构信息
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland.
出版信息
In Vivo. 2020 Nov-Dec;34(6):3263-3270. doi: 10.21873/invivo.12163.
BACKGROUND/AIM: Comparison of the expression of Ki-67, MCM3, 5, 7 and MTI/II proteins using immunohistochemistry (IHC) on whole section (WS) and tissue microarray (TMA) of laryngeal squamous cell carcinoma (LSCC) samples.
MATERIALS AND METHODS
A total of 51 archival paraffin blocks of LSCC were used. TMAs were prepared from 1.5 mm core punches. IHC reactions were performed using antibodies against Ki-67, minichromosome maintenance proteins (MCM3, 5, 7) and metallothionein (MTI/II).
RESULTS
Spearman rank correlation test revealed moderate positive correlation in the case of Ki-67: WS vs. TMA (r=0.38, p=0.07) and strong positive correlation in regard to the rest of tested markers: MCM3, WS vs. TMA (r=0.49, p=0.0004); MCM5, WS vs. TMA (r=0.61, p<0.0001); MCM7, WS vs. TMA (r=0.59, p<0.0001); MTI/II, WS vs. TMA (r=0.66, p<0.0001). Mann Whitney U-test showed no significant differences in the case of Ki-67 and MCM5. Moreover, Bland-Altman test showed a low level of bias in regard to Ki-67, WS vs. TMA and MCM5, WS vs. TMA.
CONCLUSION
TMA may be an effective and reliable method of assessment of Ki-67 and MCM5 expression in LSCC.
背景/目的：比较喉鳞状细胞癌（LSCC）标本的全切片（WS）和组织微阵列（TMA）中 Ki-67、MCM3、5、7 和 MTI/II 蛋白的表达，采用免疫组织化学（IHC）法。
材料和方法
使用了 51 个存档的 LSCC 石蜡块。TMA 由 1.5mm 核心打孔器制备。使用针对 Ki-67、微小染色体维持蛋白（MCM3、5、7）和金属硫蛋白（MTI/II）的抗体进行 IHC 反应。
结果
Spearman 等级相关检验显示，Ki-67 在 WS 与 TMA 之间存在中度正相关（r=0.38，p=0.07），在其余测试标记物中存在强正相关：MCM3，WS 与 TMA 之间（r=0.49，p=0.0004）；MCM5，WS 与 TMA 之间（r=0.61，p<0.0001）；MCM7，WS 与 TMA 之间（r=0.59，p<0.0001）；MTI/II，WS 与 TMA 之间（r=0.66，p<0.0001）。Mann Whitney U 检验显示 Ki-67 和 MCM5 之间无显著差异。此外，Bland-Altman 检验显示 Ki-67、WS 与 TMA 以及 MCM5、WS 与 TMA 之间的偏差水平较低。
结论
TMA 可能是评估 LSCC 中 Ki-67 和 MCM5 表达的有效可靠方法。
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