鸢尾素/FNDC5 在乳腺癌中的表达。
Expression of Irisin/FNDC5 in Breast Cancer.
机构信息
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Department of Pathology, Polish Mother Memorial Hospital-Research Institute, 93-338 Lodz, Poland.
出版信息
Int J Mol Sci. 2022 Mar 24;23(7):3530. doi: 10.3390/ijms23073530.
Irisin is a myokine formed from fibronectin type III domain-containing protein 5 (FNDC5), which can be found in various cancer tissues. FNDC5 and irisin levels have been poorly studied in the tumor tissues of breast cancer (BC). The aim of this study was to determine the levels of irisin expression in BC tissues and compare them to clinicopathological factors and Ki-67 and PGC-1α expression levels. Tissue microarrays (TMAs) with 541 BC tissues and 61 samples of non-malignant breast disease (NMBD; control) were used to perform immunohistochemical reactions. gene expression was measured in 40 BC tissue samples, 40 samples from the cancer margin, and 16 NMBD samples. RT-PCR was performed for the detection of gene expression. Higher irisin expression was found in BC patients compared to normal breast tissue. FNDC5/irisin expression was higher in patients without lymph node metastases. Longer overall survival was observed in patients with higher irisin expression levels. FNDC5/irisin expression was increased in BC tissues and its high level was a good prognostic factor for survival in BC patients.
鸢尾素是一种由纤维连接蛋白 III 型结构域蛋白 5（FNDC5）形成的肌因子，可在各种癌症组织中发现。FNDC5 和鸢尾素水平在乳腺癌（BC）的肿瘤组织中研究甚少。本研究旨在确定 BC 组织中鸢尾素表达水平，并将其与临床病理因素以及 Ki-67 和 PGC-1α表达水平进行比较。使用 541 例 BC 组织和 61 例非恶性乳腺疾病（NMBD；对照）组织的组织微阵列（TMA）进行免疫组织化学反应。在 40 例 BC 组织样本、40 例癌旁组织样本和 16 例 NMBD 样本中测量了 基因表达。进行 RT-PCR 检测 基因表达。与正常乳腺组织相比，BC 患者的鸢尾素表达水平更高。无淋巴结转移的患者 FNDC5/鸢尾素表达更高。鸢尾素表达水平较高的患者总生存时间更长。FNDC5/鸢尾素在 BC 组织中表达增加，其高水平是 BC 患者生存的良好预后因素。
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