Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea.
J Microbiol Biotechnol. 2021 Jan 28;31(1):104-114. doi: 10.4014/jmb.2006.06023.
Petroleum-contaminated soil is considered among the most important potential anthropogenic atmospheric methane sources. Additionally, various rhizoremediation factors can affect methane emissions by altering soil ecosystem carbon cycles. Nonetheless, greenhouse gas emissions from soil have not been given due importance as a potentially relevant parameter in rhizoremediation techniques. Therefore, in this study we sought to investigate the effects of different plant and soil amendments on both remediation efficiencies and methane emission characteristics in dieselcontaminated soil. An indoor pot experiment consisting of three plant treatments (control, maize, tall fescue) and two soil amendments (chemical nutrient, compost) was performed for 95 days. Total petroleum hydrocarbon (TPH) removal efficiency, dehydrogenase activity, and (, an alkane compound-degrading enzyme) gene abundance were the highest in the tall fescue and maize soil system amended with compost. Compost addition enhanced both the overall remediation efficiencies, as well as pmoA (, a methane-oxidizing enzyme) gene abundance in soils. Moreover, the potential methane emission of diesel-contaminated soil was relatively low when maize was introduced to the soil system. After microbial community analysis, various TPH-degrading microorganisms () and methane-oxidizing microorganisms () were observed in the rhizosphere soil. The effects of major rhizoremediation factors on soil remediation efficiency and greenhouse gas emissions discussed herein are expected to contribute to the development of sustainable biological remediation technologies in response to global climate change.
受石油污染的土壤被认为是最重要的人为大气甲烷潜在源之一。此外，各种根际修复因子可以通过改变土壤生态系统碳循环来影响甲烷排放。然而，土壤温室气体排放并没有作为根际修复技术中一个潜在的相关参数得到应有的重视。因此，在这项研究中，我们试图研究不同植物和土壤改良剂对柴油污染土壤的修复效率和甲烷排放特性的影响。进行了为期 95 天的室内盆栽实验，包括三种植物处理（对照、玉米、高羊茅）和两种土壤改良剂（化学养分、堆肥）。在添加堆肥的高羊茅和玉米土壤系统中，总石油烃（TPH）去除效率、脱氢酶活性和（烷烃化合物降解酶）基因丰度最高。添加堆肥不仅提高了整体修复效率，还提高了土壤中的 pmoA（一种甲烷氧化酶）基因丰度。此外，当向土壤系统中引入玉米时，柴油污染土壤的潜在甲烷排放量相对较低。在微生物群落分析之后，在根际土壤中观察到了各种 TPH 降解微生物（）和甲烷氧化微生物（）。本文讨论了主要根际修复因子对土壤修复效率和温室气体排放的影响，预计将有助于开发应对全球气候变化的可持续生物修复技术。
