Newell D G, Roath S, Smith J L
Br J Haematol. 1976 Mar;32(3):309-16. doi: 10.1111/j.1365-2141.1976.tb00934.x.
A study of the effects of various sample preparation techniques for scanning electron microscopy has been undertaken in an attempt to resolve conflicting descriptions of the surface topography of human peripheral blood lymphocytes. By fixing cells in suspension--a technique thought most likely to avoid the production of artefacts--no clearly defined morphological classification of lymphocytes could be made, and when T- and B-lymphocyte enriched preparations were studied their surfaces appeared similar. Both T- and B-rosetted cells showed identical morphological changes as a result of their interaction with red blood cells. The smooth cells described in other reports were found only under certain conditions of preparation. It is therefore not possible to distinguish between T- and B-cell populations, using the S.E.M., on the basis of surface morphology alone.
为了澄清有关人类外周血淋巴细胞表面形貌的相互矛盾的描述，对扫描电子显微镜的各种样品制备技术的效果进行了一项研究。通过将细胞固定在悬浮液中——这是一种最有可能避免产生假象的技术——无法对淋巴细胞进行明确的形态学分类，并且在研究富含T淋巴细胞和B淋巴细胞的制剂时，它们的表面看起来相似。T淋巴细胞和B淋巴细胞玫瑰花环形成细胞与红细胞相互作用后均表现出相同的形态变化。其他报告中描述的光滑细胞仅在某些制备条件下才能发现。因此，仅根据表面形态，使用扫描电子显微镜无法区分T细胞群和B细胞群。
