Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore, Singapore.
Singapore Institute of Manufacturing Technology, Agency for Science, Technology and Research, Singapore, Singapore.
Biotechnol Bioeng. 2021 Feb;118(2):542-554. doi: 10.1002/bit.27620. Epub 2020 Nov 17.
Spheroid culture provides cells with a three-dimensional environment that can better mimic physiological conditions compared to monolayer culture. Technologies involved in the generation of cell spheroids are continuously being innovated to produce spheroids with enhanced properties. In this paper, we review the manufacturing capabilities of current cell spheroid generation technologies. We propose that spheroid generation technologies should enable tight and robust process controls to produce spheroids of consistent and repeatable quality. Future technology development for the generation of cell spheroids should look into improvement in process control, standardization, scalability and monitoring, in addition to advanced methods of spheroid transfer and characterization.
球状体培养为细胞提供了三维环境，相比于单层培养，能更好地模拟生理条件。用于生成细胞球状体的技术在不断创新，以生产出具有增强性能的球状体。在本文中，我们综述了当前细胞球状体生成技术的制造能力。我们提出，球状体生成技术应能够实现严格而稳健的过程控制，以生产出具有一致和可重复质量的球状体。未来细胞球状体生成技术的发展除了先进的球状体转移和表征方法外，还应着眼于改进过程控制、标准化、可扩展性和监测。
