保持冰晶中的结构信息：冷冻电镜/电子断层扫描工作流程优化。

Maintaining Context in Ice: Cryo-EM/ET Workflow Optimizations.

机构信息

Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Structure. 2020 Nov 3;28(11):1179-1181. doi: 10.1016/j.str.2020.10.004.

DOI:10.1016/j.str.2020.10.004

Abstract

In this issue of Structure, breakthroughs in cryo-EM/ET research are presented. Klebl et al. (2020) demonstrate how speed in sample vitrification impacts the quality of macromolecular particles in resultant cryo-EM grids. Wu et al. (2020) combine fluorescence, ion beam milling, and tomography to unravel unique features in vitrified yeast cells.

摘要

本期《结构》介绍了冷冻电镜/电子断层扫描研究的突破。Klebl 等人（2020 年）展示了样品的快速冷冻如何影响冷冻电镜网格中大分子颗粒的质量。Wu 等人（2020 年）结合荧光、离子束铣削和断层扫描技术，揭示了冷冻酵母细胞的独特特征。