Department of Veterinary Medicine, Veterinary Teaching Hospital, University of Milan, Lodi, Italy.
Department of Veterinary Sciences, Veterinary Teaching Hospital, Univeristy of Pisa, Pisa, Italy.
BMC Vet Res. 2020 Nov 4;16(1):422. doi: 10.1186/s12917-020-02629-4.
Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240 h after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS).
Compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24 h post-infusion, with lowest values observed between 3 to 7 days post-infusion, followed by a normalisation to pre-infusion levels the tenth day.
The results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.
对氧磷酶-1（PON-1）是一种抗氧化酶，其活性在许多物种的急性期反应中降低。关于 PON-1 及其在马败血症炎症中作为负急性期蛋白的作用知之甚少，但最近有研究发现其在诊断 SIRS 和预测患病马预后方面具有很大的应用价值。本研究旨在研究实验性内毒素血症后马 PON-1 的行为。为此，对 66 份来自 6 匹临床健康母马的血浆样本进行了 PON-1 活性测量，这些母马先前被纳入另一项研究，在静脉注射大肠杆菌 O55:B5 脂多糖（LPS）后 12 至 240 小时之间的多个时间点进行了测量。
与基线值相比，从输注后 24 小时开始观察到 PON-1 活性逐渐短暂下降，最低值出现在输注后 3 至 7 天之间，然后在第 10 天恢复到输注前水平。
本研究结果表明，PON-1 活性的测量和监测可能有助于评估马内毒素血症的进展和恢复。需要在自然发生败血症的马中进行进一步的研究。
