重复采样提高了六岁以下儿童疟疾显微镜检查的灵敏度。
Repeated sampling improved the sensitivity of malaria microscopy in children under six years.
作者信息
Aninagyei Enoch
机构信息
Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, PMB 31, Ho, Volta Region, Ghana.
出版信息
BMC Res Notes. 2020 Nov 4;13(1):508. doi: 10.1186/s13104-020-05359-w.
Abstract
OBJECTIVE
Microscopy remains the gold standard for identification of malaria parasites. However, the sensitivity of malaria microscopy is low. This study evaluated the impact of repeated sampling up to 12 h in 177 children < 6 years with suspected malaria.
RESULTS
The median age was 3 years (interquartile range, 2.0-4.0 years). Eighty-nine percent (158/177) presented with hyperthermia together with one or more of the following symptoms: chills, headache, sweating, fatigue, nausea, abdominal pain, vomiting, diarrhea and cough. Baseline microscopy confirmed malaria in 29.9% (53/177) of the suspects. Repeated testing at 6 and 12 h increased the positive detection rates to 35.0% (62/177) and 41.8% (74/177), respectively. Microscopy underestimated malaria diagnosis by 11.9% on single testing. Children showing classical signs of malaria but with initial negative parasitological reports should be retested between 6 to 12 h to confirm or rule out a diagnosis of malaria.
摘要
目的
显微镜检查仍然是疟原虫鉴定的金标准。然而，疟疾显微镜检查的敏感性较低。本研究评估了对177名6岁以下疑似疟疾儿童进行长达12小时的重复采样的影响。
结果
中位年龄为3岁（四分位间距，2.0 - 4.0岁）。89%（158/177）的儿童出现高热，并伴有以下一种或多种症状：寒战、头痛、出汗、疲劳、恶心、腹痛、呕吐、腹泻和咳嗽。基线显微镜检查确诊29.9%（53/177）的疑似患者患有疟疾。在6小时和12小时进行重复检测后，阳性检出率分别提高到35.0%（62/177）和41.8%（74/177）。单次检测时，显微镜检查对疟疾诊断的低估率为11.9%。对于表现出疟疾典型症状但初始寄生虫学报告为阴性的儿童，应在6至12小时内进行重新检测，以确认或排除疟疾诊断。
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