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1
Protein engineering. The design, synthesis and characterization of factitious proteins.蛋白质工程。人工合成蛋白质的设计、合成与表征。
Biochem J. 1987 Aug 15;246(1):1-17. doi: 10.1042/bj2460001.
2
Catalytic contributions from remote regions of enzyme structure.酶结构远端区域的催化作用。
Chem Rev. 2011 Dec 14;111(12):7595-624. doi: 10.1021/cr100042n. Epub 2011 Sep 19.
3
A novel crystal form of pyrrolysyl-tRNA synthetase reveals the pre- and post-aminoacyl-tRNA synthesis conformational states of the adenylate and aminoacyl moieties and an asparagine residue in the catalytic site.吡咯赖氨酸-tRNA合成酶的一种新型晶体形式揭示了腺苷酸和氨酰基部分以及催化位点中天冬酰胺残基在氨酰基-tRNA合成前后的构象状态。
Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):5-15. doi: 10.1107/S0907444912039881. Epub 2012 Dec 20.
4
[Functional and evolutionary aspects of the aminoacyl-tRNA synthetases].[氨酰-tRNA合成酶的功能与进化方面]
Rev Latinoam Microbiol. 1991 Jan-Mar;33(1):87-101.
5
Structural diversity and protein engineering of the aminoacyl-tRNA synthetases.氨酰-tRNA 合成酶的结构多样性与蛋白质工程。
Biochemistry. 2012 Nov 6;51(44):8705-29. doi: 10.1021/bi301180x. Epub 2012 Oct 26.
6
Distinct kinetic mechanisms of the two classes of Aminoacyl-tRNA synthetases.两类氨酰-tRNA合成酶的不同动力学机制。
J Mol Biol. 2006 Aug 11;361(2):300-11. doi: 10.1016/j.jmb.2006.06.015. Epub 2006 Jun 27.
7
[Gene duplication and protein evolution. Case of aminoacyl-t-RNA synthetases].
C R Seances Soc Biol Fil. 1977;171(2):272-9.
8
Sequence, structural and evolutionary relationships between class 2 aminoacyl-tRNA synthetases.2类氨酰-tRNA合成酶之间的序列、结构及进化关系。
Nucleic Acids Res. 1991 Jul 11;19(13):3489-98. doi: 10.1093/nar/19.13.3489.
9
Aminoacylation of RNA minihelices: implications for tRNA synthetase structural design and evolution.RNA小螺旋的氨酰化:对氨酰tRNA合成酶结构设计和进化的启示
Crit Rev Biochem Mol Biol. 1993;28(4):309-22. doi: 10.3109/10409239309078438.
10
Sequence comparisons in the aminoacyl-tRNA synthetases with emphasis on regions of likely homology with sequences in the Rossmann fold in the methionyl and tyrosyl enzymes.氨酰-tRNA合成酶中的序列比较,重点关注与甲硫氨酰和酪氨酰酶中罗斯曼折叠序列可能存在同源性的区域。
Protein Seq Data Anal. 1988 Feb;1(3):187-93.

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1
The Fusion Multistage Synthetic Peptides as the Best Candidates for New Tuberculosis Vaccine.融合多阶段合成肽作为新型结核病疫苗的最佳候选物。
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2
Protein dynamics and motions in relation to their functions: several case studies and the underlying mechanisms.蛋白质动力学及其与功能的关系:几个案例研究及其潜在机制。
J Biomol Struct Dyn. 2014;32(3):372-93. doi: 10.1080/07391102.2013.770372. Epub 2013 Mar 25.
3
(1)H, (13)C and (15)N NMR backbone assignments of the 269-residue serine protease PB92 from Bacillus alcalophilus.(1)来源于嗜碱性芽孢杆菌的 269 残基丝氨酸蛋白酶 PB92 的(1)H、(13)C 和(15)N NMR 骨架归属。
J Biomol NMR. 1994 Jan;4(1):123-8. doi: 10.1007/BF00178340.
4
The effect of calciums on molecular motions of proteinase K.钙对蛋白酶 K 分子运动的影响。
J Mol Model. 2011 Feb;17(2):289-300. doi: 10.1007/s00894-010-0724-6. Epub 2010 May 6.
5
Insights derived from molecular dynamics simulation into the molecular motions of serine protease proteinase K.从分子动力学模拟中获得的丝氨酸蛋白酶蛋白酶 K 的分子运动见解。
J Mol Model. 2010 Jan;16(1):17-28. doi: 10.1007/s00894-009-0518-x. Epub 2009 May 23.
6
Isolation of suppressors of temperature-sensitive folding mutations.
J Bacteriol. 1994 Jan;176(1):137-42. doi: 10.1128/jb.176.1.137-142.1994.
7
De novo and inverse folding predictions of protein structure and dynamics.蛋白质结构与动力学的从头预测和反向折叠预测。
J Comput Aided Mol Des. 1993 Aug;7(4):397-438. doi: 10.1007/BF02337559.
8
Complete 1H, 13C and 15N NMR assignments and secondary structure of the 269-residue serine protease PB92 from Bacillus alcalophilus.
J Biomol NMR. 1995 Apr;5(3):259-70. doi: 10.1007/BF00211753.
9
Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.利用天然结构变异体和反应性探针动力学对有助于酶催化的相互作用进行超晶体学分辨率研究。
Biochem J. 1988 Dec 1;256(2):543-58. doi: 10.1042/bj2560543.
10
beta-lactamase I from Bacillus cereus. Structure and site-directed mutagenesis.来自蜡样芽孢杆菌的β-内酰胺酶I。结构与定点诱变
Biochem J. 1987 Dec 15;248(3):657-62. doi: 10.1042/bj2480657.

本文引用的文献

1
Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering.通过蛋白质工程探究位阻和疏水性效应对酶-底物相互作用的影响。
Science. 1986 Aug 8;233(4764):659-63. doi: 10.1126/science.233.4764.659.
2
Three-dimensional structure of ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum at 2.9 A resolution.红假单胞菌核酮糖-1,5-二磷酸羧化酶/加氧酶的三维结构,分辨率为 2.9 A。
EMBO J. 1986 Dec 20;5(13):3409-15. doi: 10.1002/j.1460-2075.1986.tb04662.x.
3
The conformations of hirudin in solution: a study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics.水蛭素在溶液中的构象:一项利用核磁共振、距离几何和受限分子动力学的研究。
EMBO J. 1987 Feb;6(2):529-37. doi: 10.1002/j.1460-2075.1987.tb04785.x.
4
Determination and analysis of the 2 A-structure of copper, zinc superoxide dismutase.铜锌超氧化物歧化酶2 A结构的测定与分析
J Mol Biol. 1982 Sep 15;160(2):181-217. doi: 10.1016/0022-2836(82)90174-7.
5
Tyrosyl-tRNA synthetase forms a mononucleotide-binding fold.酪氨酰 - tRNA合成酶形成一种单核苷酸结合结构域。
J Mol Biol. 1982 Jul 15;158(4):699-709. doi: 10.1016/0022-2836(82)90255-8.
6
Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.丝氨酸蛋白酶四面体中间体中天冬氨酸-102和组氨酸-57质子化状态的直接测定:胰蛋白酶的中子结构
Biochemistry. 1981 Oct 27;20(22):6462-74. doi: 10.1021/bi00525a027.
7
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.寡核苷酸定向诱变作为研究蛋白质功能的一种通用且强大的方法。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6409-13. doi: 10.1073/pnas.79.21.6409.
8
Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.硫醇β-内酰胺酶:RTEMβ-内酰胺酶活性位点的丝氨酸被半胱氨酸残基取代。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7157-60. doi: 10.1073/pnas.79.23.7157.
9
Electrostatic recognition between superoxide and copper, zinc superoxide dismutase.超氧化物与铜锌超氧化物歧化酶之间的静电识别
Nature. 1983;306(5940):287-90. doi: 10.1038/306287a0.
10
Mutation of antitrypsin to antithrombin. alpha 1-antitrypsin Pittsburgh (358 Met leads to Arg), a fatal bleeding disorder.抗胰蛋白酶突变为抗凝血酶。α1-抗胰蛋白酶匹兹堡型(358位甲硫氨酸突变为精氨酸),一种致命的出血性疾病。
N Engl J Med. 1983 Sep 22;309(12):694-8. doi: 10.1056/NEJM198309223091203.

Protein engineering. The design, synthesis and characterization of factitious proteins.

作者信息

Shaw W V

机构信息

Department of Biochemistry, University of Leicester, U.K.

出版信息

Biochem J. 1987 Aug 15;246(1):1-17. doi: 10.1042/bj2460001.

DOI:10.1042/bj2460001
PMID:3314863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148234/
Abstract
摘要