Atalayin Ozkaya Cigdem, Tezel Huseyin, Armagan Guliz, Tuzcu Fulya, Sahbaz Ufuk, Dagci Taner
Department of Restorative Dentistry, Ege University School of Dentistry.
Department of Biochemistry, Ege University Faculty of Pharmacy.
J Oral Sci. 2020 Dec 23;63(1):46-49. doi: 10.2334/josnusd.20-0034. Epub 2020 Nov 4.
The present study was conducted to determine oxidative stress and cell viability after contact with resin composites polymerized for different times.
Disk-shaped specimens of Admira Fusion, Ceram X One Universal, Solare x and Filtek Z550 (n = 12) were prepared, and two subgroups with polymerization times of 20 and 40 s were employed. The specimens were incubated with mouse fibroblast cells for 48 and 72 h, and changes in reactive oxygen species (ROS) production and cellular viability were determined by an assay with a cell-permeable fluorescent dye, 2',7'-dichlorodihydrofluorescein diacetate (HDCF-DA), and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, respectively.
At 72 h, ROS production in the presence of Admira Fusion polymerized for 40 s was reduced relative to that in the presence of Admira Fusion polymerized for 20 s (P < 0.05). Cell viability was maximal in the Admira Fusion and Solare x groups and there was no difference relative to the control group at 48 h. Cell viability was higher in the Admira Fusion and Solare x groups polymerized for 40 s than for the same materials polymerized for 20 s at 72 h (P < 0.05).
Extension of the polymerizaton time has a material-specific effect and may be used as a strategy to increase the biocompability of resin composites.
本研究旨在确定与不同聚合时间的树脂复合材料接触后的氧化应激和细胞活力。
制备了Admira Fusion、Ceram X One Universal、Solare x和Filtek Z550的圆盘形标本（n = 12），并采用了聚合时间为20秒和40秒的两个亚组。将标本与小鼠成纤维细胞孵育48小时和72小时，分别通过使用细胞可渗透荧光染料2',7'-二氯二氢荧光素二乙酸酯（HDCF-DA）的检测和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测来测定活性氧（ROS）产生和细胞活力的变化。
在72小时时，相对于聚合20秒的Admira Fusion，聚合40秒的Admira Fusion存在时的ROS产生减少（P < 0.05）。在48小时时，Admira Fusion和Solare x组的细胞活力最高，与对照组相比无差异。在72小时时，聚合40秒的Admira Fusion和Solare x组的细胞活力高于相同材料聚合20秒时的细胞活力（P < 0.05）。
延长聚合时间具有材料特异性效应，可作为提高树脂复合材料生物相容性的一种策略。
