Oral Health Prev Dent. 2023 Jul 13;21:243-250. doi: 10.3290/j.ohpd.b4211055.
This study aimed to compare the cell toxicity and biological characteristics of Ketac GIC (glass-ionomer cement), Nexus RMGIC (resin-modified glass-ionomer cement), and RelyX RC (resin cement) in human periodontal stem cells (PDSCs).
To compare the effects of Ketac GIC, Nexus RMGIC, and RelyX RC on PDSCs, the cements were diluted from 1:2 to 1:8. PDSCs were then treated with the serially diluted cements with or without N-acetyl-cysteine (NAC), and cell survival was measured using water-soluble tetrazolium salt (WST-1) assay. Intracellular reactive oxygen species (ROS) was measured using 2',7'-dichlorofluorescin diacetate (DCFDA), and western blot analysis was performed to observe phosphorylation and activation of extracellular signal-regulated kinase (ERK) by Nexus RMGIC or RelyX RC.
Cell death and proliferation were dose-dependently reduced following Nexus RMGIC or RelyX RC treatment. In addition, Nexus RMGIC or RelyX RC showed an increase intracellular ROS generation compared to Ketac GIC. Pretreatment with NAC confirmed the suppression of cell toxicity and ROS generation induced by Nexus RMGIC or RelyX RC. Nexus RMGIC or RelyX RC activates ERK phosphorylation, not p38 phosphorylation, in PDSCs.
This study showed that the treatment with Nexus RMGIC or RelyX generates intracellular ROS and cell death through the ERK signaling pathway in PDSCs. In contrast, these effects were not observed with Ketac GIC, indicating that resin-based materials may have cytotoxic and genotoxic effects on PDSCs.
本研究旨在比较 Ketac GIC（玻璃离子水门汀）、Nexus RMGIC（树脂改良型玻璃离子水门汀）和 RelyX RC（树脂粘接剂）对人牙周干细胞（PDSCs）的细胞毒性和生物学特性。
为了比较 Ketac GIC、Nexus RMGIC 和 RelyX RC 对 PDSCs 的影响，将这些水门汀从 1:2 稀释至 1:8。然后用连续稀释的水门汀处理 PDSCs，同时或不使用 N-乙酰半胱氨酸（NAC），并使用水溶性四唑盐（WST-1）测定法测量细胞存活率。使用 2',7'-二氯荧光素二乙酸酯（DCFDA）测量细胞内活性氧（ROS），并用 Western blot 分析观察 Nexus RMGIC 或 RelyX RC 对细胞外信号调节激酶（ERK）的磷酸化和激活。
Nexus RMGIC 或 RelyX RC 处理后，细胞死亡和增殖呈剂量依赖性下降。此外，与 Ketac GIC 相比，Nexus RMGIC 或 RelyX RC 显示出细胞内 ROS 生成增加。用 NAC 预处理证实了 Nexus RMGIC 或 RelyX RC 诱导的细胞毒性和 ROS 生成的抑制。Nexus RMGIC 或 RelyX RC 激活 PDSCs 中的 ERK 磷酸化，而不是 p38 磷酸化。
本研究表明，Nexus RMGIC 或 RelyX RC 处理会通过 PDSCs 中的 ERK 信号通路产生细胞内 ROS 和细胞死亡。相比之下，Ketac GIC 则没有观察到这些影响，这表明树脂基材料可能对 PDSCs 具有细胞毒性和遗传毒性作用。
