Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.
Faculty of Acupuncture and Tuina, Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
Biosci Rep. 2019 Mar 22;39(3). doi: 10.1042/BSR20181734. Print 2019 Mar 29.
Skin wound, a common form of skin damage in daily life, remains a serious challenge in clinical treatment. Bioactive peptides with high efficiency have been considered as potential therapeutic candidates for wound healing. In this report, a novel short linear peptide, with mature peptide sequence of 'GLLSGINAEWPC' and no obvious similarity with other known bioactive peptides, was identified by genomic method from the skin of odorous frog, Our results suggested that OA-GL12 (OA: abbreviation of species (), GL: two initial amino acids, 12: peptide length) obviously accelerated the scratch-healing of keratinocytes and human fibroblasts in a time- and concentration-dependent manner. Meanwhile, OA-GL12 showed significant effect in promoting the wound healing on the full-thickness skin wound model. Inflammatory assay results demonstrated that OA-GL12 induced the secretion of tumor necrosis factor (TNF) and transforming growth factor β1 (TGF-β1) on murine macrophage cell line (RAW264.7), which might explain the powerful accelerating capacity of wound healing. Moreover, results also indicated that epidermal growth factor receptor (EGFR) was involved in the mechanisms underlying the scratch-healing promoting activity of OA-GL12. In addition, OA-GL12 showed obvious free radical scavenging activity. Results supported that OA-GL12 did not exert risk in acute toxicity, hemolytic activity, and direct antibacterial activity. The remarkable effect of OA-GL12 on promoting wound healing verified in this research made it potential to be a novel template for the development of wound healing-promoting agents.
皮肤创伤是日常生活中常见的皮肤损伤形式，在临床治疗中仍然是一个严重的挑战。具有高效的生物活性肽已被认为是伤口愈合的潜在治疗候选物。在本报告中，通过基因组方法从臭蛙皮肤中鉴定出一种新型短线性肽，其成熟肽序列为“GLLSGINAEWPC”，与其他已知的生物活性肽没有明显的相似性。我们的结果表明，OA-GL12（OA：物种缩写（），GL：两个初始氨基酸，12：肽长度）明显以时间和浓度依赖的方式加速角质形成细胞和人成纤维细胞的划痕愈合。同时，OA-GL12 在促进全层皮肤创伤模型的伤口愈合方面显示出显著效果。炎症测定结果表明，OA-GL12 诱导肿瘤坏死因子（TNF）和转化生长因子 β1（TGF-β1）在鼠巨噬细胞系（RAW264.7）中的分泌，这可能解释了其强大的促进伤口愈合能力。此外，结果还表明表皮生长因子受体（EGFR）参与了 OA-GL12 促进划痕愈合活性的机制。此外，OA-GL12 表现出明显的自由基清除活性。结果表明，OA-GL12 在外生性急性毒性、溶血活性和直接抑菌活性方面没有风险。本研究中证实的 OA-GL12 对促进伤口愈合的显著效果使其有可能成为开发促进伤口愈合剂的新型模板。
