Rubtsova M Iu, Cherednikova T V, Susova M B, Gavrilova E M, Egorov A M
Biokhimiia. 1987 Sep;52(9):1531-8.
The interaction of monoclonal antibodies of three types with the ATP-labeled insulin dimer was studied by the luminescent immunocofactor method. It was shown that the effective equilibrium binding constant increases at equimolar antigen/antibody concentrations. This can be due to the formation of multimolecular complexes between the antigens and antibodies. The feasibility of the binding constants increase during the formation of cyclic tetramolecular complexes is considered. A theoretical model for the description of interaction between the bivalent antigen and antibodies based on the increase of the binding constant during the formation of cyclic complexes is proposed. The coefficients of binding constant increase for antigens belonging to three different clones were calculated.
采用发光免疫辅因子法研究了三种类型的单克隆抗体与ATP标记的胰岛素二聚体之间的相互作用。结果表明，在等摩尔抗原/抗体浓度下，有效平衡结合常数会增加。这可能是由于抗原与抗体之间形成了多分子复合物。考虑了在环状四分子复合物形成过程中结合常数增加的可行性。提出了一个基于环状复合物形成过程中结合常数增加来描述二价抗原与抗体之间相互作用的理论模型。计算了属于三个不同克隆的抗原的结合常数增加系数。
