Khan-Dawood F S
Department of Obstetrics and Gynecology, University of Illinois College of Medicine, Chicago 60612.
Biol Reprod. 1987 Oct;37(3):659-64. doi: 10.1095/biolreprod37.3.659.
Recently, using a highly specific radioimmunoassay, we have demonstrated that the concentration of oxytocin in the corpus luteum of the human and cynomolgus monkey are several fold higher than in the peripheral circulation. In this study, we have examined the corpora lutea and ovarian stroma from the ovaries of normal adult cycling baboons (Papio anubis) for the presence of oxytocin through the use of immunocytochemical procedures. Tissues obtained at laparotomy were fixed in Bouin's solution and embedded in paraffin; immunoreactive oxytocin was localized with peroxidase-antiperoxidase and 3.3' diaminobenzidine. Six corpora lutea with stroma were obtained--two each from the early (Day 14-20), mid-(Day 21-24), and late (Day 25-30) stages of the luteal phase. Immunoreactive oxytocin was localized in all corpora lutea examined but was absent from all stroma samples. Larger areas of the corpus luteum from the mid-luteal phase showed staining for oxytocin, and the intensity of staining for this peptide was maximal in this phase of the cycle.
最近，我们使用一种高度特异的放射免疫分析法证明，人类和食蟹猴黄体中催产素的浓度比外周循环中的浓度高几倍。在本研究中，我们通过免疫细胞化学方法检查了正常成年周期性狒狒（埃及狒狒）卵巢中的黄体和卵巢基质中是否存在催产素。剖腹手术获取的组织用布因氏液固定并石蜡包埋；用过氧化物酶-抗过氧化物酶和3,3'-二氨基联苯胺定位免疫反应性催产素。获得了六个带有基质的黄体——分别来自黄体期的早期（第14 - 20天）、中期（第21 - 24天）和晚期（第25 - 30天），各两个。免疫反应性催产素在所有检查的黄体中均有定位，但在所有基质样本中均未发现。来自黄体中期的黄体有更大面积显示催产素染色，且该肽的染色强度在周期的这个阶段最大。
