Carpenter M B, Chang L, Pereira A B, Hersh L B
Department of Anatomy, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
Brain Res. 1987 Aug 25;418(2):403-8. doi: 10.1016/0006-8993(87)90112-0.
Immunocytochemical studies of the brainstem were done in the squirrel monkey and rat using the same polyclonal antisera for choline acetyltransferase (ChAT). Cells immunoreactive for ChAT (ChATir) were evident in large numbers in visceral and motor cranial nerve nuclei in both species, but virtually no ChATir cells were seen in the vestibular nuclear complex of the rat. In the monkey ChATir cells were distributed in caudal parts of the medial (MVN) and in dorsal parts of the inferior (IVN) vestibular nuclei. Only a few immunoreactive cells were seen in the rostral MVN and none were found in cell group f of the IVN. Nearly all cells of group z and x, which do not receive primary vestibular afferents, were immunoreactive to ChAT. None of the cells in the superior and lateral vestibular nuclei, cell group y, the infracerebellar nucleus or the interstitial nucleus of the vestibular nerve were immunoreactive for ChAT. Cells immunoreactive to ChAT were present in large numbers in the rostral part of the nucleus prepositus in the monkey, but not in the rat. The relatively small number and distribution of ChATir cells in the MVN suggested they could constitute only a small fraction of the MVN neurons that contribute to a massive commissural system. Significant differences in cholinergic vestibular neurons appear to exist between the rat and the monkey.
利用相同的抗胆碱乙酰转移酶（ChAT）多克隆抗血清，对松鼠猴和大鼠的脑干进行了免疫细胞化学研究。在这两个物种的内脏和运动性脑神经核中，大量对ChAT免疫反应阳性（ChATir）的细胞很明显，但在大鼠的前庭核复合体中几乎未见ChATir细胞。在猴子中，ChATir细胞分布在内侧前庭核（MVN）的尾部和下前庭核（IVN）的背侧部分。在前庭内侧核头端仅见少数免疫反应阳性细胞，在下前庭核的细胞群f中未发现阳性细胞。几乎所有不接受前庭初级传入纤维的z组和x组细胞对ChAT均呈免疫反应阳性。在上前庭核、外侧前庭核、细胞群y、小脑下核或前庭神经间质核中，没有一个细胞对ChAT呈免疫反应阳性。在猴子中，前庭前置核头端存在大量对ChAT免疫反应阳性的细胞，而在大鼠中则没有。前庭内侧核中ChATir细胞的数量相对较少且分布情况表明，它们可能仅构成参与一个庞大连合系统的前庭内侧核神经元的一小部分。大鼠和猴子之间胆碱能前庭神经元似乎存在显著差异。
