Institute of Plant Protection, Guangdong Academy of Agricultural Sciences and Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Key Laboratory of Tea Quality and Safety Control, Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Jun;38:100798. doi: 10.1016/j.cbd.2021.100798. Epub 2021 Jan 29.
The alfalfa plant bug Adelphocoris lineolatus, an economically important pest, has representative behavioral characteristics with host plants transfer. Olfactory system is essential for insects to perceive ever-changing chemical signals in the external environment, and chemosensory genes play crucial roles in signals reception and transduction. In this work, we compared the differences in chemosensory genes expression before and after host plants transfer by constructing 12 antennal transcriptomes of male and female bugs, respectively. The results showed that the expression levels of most chemosensory genes in A. lineolatus changed to adapt to the transformation of the hosts plant. More remarkable, female bugs had more up-regulated chemosensory genes than males. Differentially expressed genes (DEGs) analysis revealed three odorant binding proteins (OBPs), three chemosensory proteins (CSPs), eight odorant receptors (ORs) and one ionotropic receptor (IR) showed significant differences when the host plant transferred. There were complex characteristics of up- and down- regulated genes in male and female adults, among which OBP19 showed higher expression in females exposing to the new host plant alfalfa, suggesting this OBP may be associated with the localization of the oviposition site. The OR54 and OR82 were up-regulated in both genders, indicating their possible roles in recognizing some alfalfa-specific volatiles. These findings will provide valuable insights in biological functions of chemosensory genes in A. lineolatus and facilitate the development of new targets for novel strategies to control the alfalfa plant bug and other herbivores.
紫穗槐蚜（Adelphocoris lineolatus）是一种具有代表性的经济重要害虫，具有寄主植物转移的行为特征。嗅觉系统对于昆虫感知外部环境中不断变化的化学信号至关重要，而化学感受基因在信号接收和转导中起着关键作用。在这项工作中，我们通过构建雄性和雌性蚜虫的 12 个触角转录组，分别比较了寄主植物转移前后化学感受基因表达的差异。结果表明，A. lineolatus 中的大多数化学感受基因的表达水平发生了变化，以适应寄主植物的转化。更显著的是，雌性蚜虫比雄性蚜虫有更多上调的化学感受基因。差异表达基因（DEGs）分析显示，当寄主植物转移时，有三个气味结合蛋白（OBPs）、三个化学感受蛋白（CSPs）、八个气味受体（ORs）和一个离子型受体（IR）表现出显著差异。雌雄成虫中存在上调和下调基因的复杂特征，其中 OBPl9 在暴露于新寄主植物紫穗槐的雌性中表达较高，表明该 OBP 可能与产卵部位的定位有关。OR54 和 OR82 在两性中均上调，表明它们可能在识别某些紫穗槐特异性挥发物方面发挥作用。这些发现将为 A. lineolatus 中化学感受基因的生物学功能提供有价值的见解，并有助于开发新的目标，以制定控制紫穗槐蚜和其他食草昆虫的新策略。
