Jansson P E, Kenne L, Wehler T
Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Carbohydr Res. 1987 Sep 1;166(2):271-82. doi: 10.1016/0008-6215(87)80063-0.
A 1H-n.m.r. study of the O-polysaccharides from different types of Shigella flexneri has been performed. With the aid of 2D-n.m.r. techniques, namely, J-resolved, spin-spin correlation, and NOESY experiments, most of the structural features of these polysaccharides could be deduced. Sequences could generally be obtained from the NOESY experiments. When using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjacent residue could be obtained, thereby giving complementary sequence information.
已对不同类型福氏志贺氏菌的O-多糖进行了¹H-核磁共振研究。借助二维核磁共振技术，即J-分解、自旋-自旋相关和核Overhauser效应光谱（NOESY）实验，可以推断出这些多糖的大部分结构特征。序列通常可从NOESY实验中获得。在这些实验中使用延长的混合时间时，可获得由于自旋扩散从一个端基质子到相邻残基的端基质子而产生的交叉峰，从而给出互补的序列信息。
