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对小黄卵泡的综合转录组分析表明，索桑多瓦锚蛋白重复结构域家族成员A抑制鸡卵泡的选择。

Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection.

作者信息

Zhong Conghao, Liu Zemin, Qiao Xibo, Kang Li, Sun Yi, Jiang Yunliang

机构信息

Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271000, China.

Shandong Jihua Poultry Breeding Co. Ltd., Rizhao 276800, China.

出版信息

Anim Biosci. 2021 Aug;34(8):1290-1302. doi: 10.5713/ajas.20.0404. Epub 2020 Oct 13.

DOI:10.5713/ajas.20.0404
PMID:33152230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8255886/
Abstract

OBJECTIVE

Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become a hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear.

METHODS

In this study, the whole transcriptome sequencing of SY follicles with different expression levels of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by quantitative real-time polymerase chain reaction. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles.

RESULTS

Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosondowah ankyrin repeat domain family member A (SOWAHA) was the common target gene of three miRNAs and one lncRNA. SOWAHA was mainly expressed in small white (SW) and SY follicles and was affected by follicle stimulation hormone (FSH) treatment in the granulosa cells. Knockdown of SOWAHA inhibited the expression of Wnt family member 4 (Wnt4) and steroidogenic acute regulatory protein (StAR) in the granulosa cells of prehierarchal follicles, while stimulated Wnt4 in hierarchal follicles. Overexpression of SOWAHA increased the expression of Wnt4 in the granulosa cells of prehierarchal follicles, decreased that of StAR and cytochrome P450 family 11 subfamily A member 1 in the granulosa cells of hierarchal follicles and inhibited the proliferation of granulosa cells.

CONCLUSION

Integrated analysis of chicken SY follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection.

摘要

目的

卵泡选择是母鸡产蛋过程中的一个重要环节。在几个小黄卵泡中，卵泡刺激素受体（FSHR）表达最高的那个卵泡将被选出来成为等级卵泡。长链非编码RNA（lncRNA）、微小RNA（miRNA）及其他非编码RNA在鸡卵泡选择中的作用尚不清楚。

方法

本研究对济宁百日鸡中FSHR表达水平不同的小黄卵泡进行了全转录组测序，并通过实时定量聚合酶链反应验证了随机选择的30个mRNA、lncRNA和miRNA的表达。对筛选出的mRNA、lncRNA、miRNA及其靶基因进行了初步研究和生物信息学分析。在鸡卵泡颗粒细胞中检测了鉴定基因的作用。

结果

对FSHR表达不同的鸡小黄卵泡进行综合转录组分析，发现467个差异表达的mRNA基因、134个差异表达的lncRNA基因和34个差异表达的miRNA基因，索桑多瓦锚蛋白重复结构域家族成员A（SOWAHA）是3个miRNA和1个lncRNA的共同靶基因。SOWAHA主要在小白卵泡和小黄卵泡中表达，在颗粒细胞中受卵泡刺激素（FSH）处理的影响。敲低SOWAHA可抑制前等级卵泡颗粒细胞中Wnt家族成员4（Wnt4）和类固醇生成急性调节蛋白（StAR）的表达，而在等级卵泡中刺激Wnt4表达。过表达SOWAHA可增加前等级卵泡颗粒细胞中Wnt4的表达，降低等级卵泡颗粒细胞中StAR和细胞色素P450家族11亚家族A成员1的表达，并抑制颗粒细胞增殖。

结论

鸡小黄卵泡转录组的综合分析确定SOWAHA是一个在卵巢卵泡颗粒细胞中受FSH影响的网络基因。SOWAHA影响参与鸡卵泡选择的基因表达并抑制颗粒细胞增殖，提示其在鸡卵泡选择中起抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/65017a1947cc/ajas-20-0404f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/fa42d55b10d3/ajas-20-0404f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/5721b82d3b3c/ajas-20-0404f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/4dec560b54fc/ajas-20-0404f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/a02968a99afc/ajas-20-0404f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/6784d12acf9a/ajas-20-0404f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/0658aa999f64/ajas-20-0404f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/65017a1947cc/ajas-20-0404f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/fa42d55b10d3/ajas-20-0404f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/fcebb719c6b4/ajas-20-0404f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/5721b82d3b3c/ajas-20-0404f3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/6784d12acf9a/ajas-20-0404f6.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df38/8255886/65017a1947cc/ajas-20-0404f8.jpg

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