High-fat diet and acute stress have different effects on object preference tests in rats during adolescence and adulthood.

Meals of high-fat diet (HFD) during adolescence produce stronger impairments to memory during adolescence than adulthood, however recovery of memory from adolescent HFD is underexplored. In addition, many tests of rodent memory are confounded by aversive or food-based stimuli, making it difficult to determine baseline memory processing affected by HFD. Thus, we utilized three cohorts of rats (adolescent HFD, adult HFD, and adolescent HFD with recovery) to explore the effects of HFD at different ages on two traditional tests of memory based strictly on object exploration, novel object recognition and novel object location tests. To isolate stress as a variable, rats were tested either at baseline or with cold water swim occurring directly after object acquisition. Results show that preference for novel objects is impaired by stress across all groups, but HFD alone only impairs preference for novel objects during adolescence, although this recovers after switching to a control diet. Additionally, preference for an object in a new location is impaired by HFD in all age groups and fails to recover following diet change. Together the data suggest that stress and HFD differentially affect object preference, based on test type, except during the adolescent period. Because these tests are traditionally interpreted as memory processes dependent on two distinct brain regions, the hippocampus and perirhinal cortex, these results support that stress and HFD affect the hippocampus and perirhinal cortex differently. The data affirm that while perirhinal cortex-dependent behavior recovers, the adolescent period is susceptible to long-lasting dysfunctions of hippocampal behavior by HFD.