Department of Synthetic Organic Chemistry, Institute for Molecules and Materials, Radboud University, Nijmegen, the Netherlands.
Department of Synthetic Organic Chemistry, Institute for Molecules and Materials, Radboud University, Nijmegen, the Netherlands.
Curr Opin Chem Biol. 2021 Feb;60:79-88. doi: 10.1016/j.cbpa.2020.09.002. Epub 2020 Nov 3.
The emergence of bioorthogonal reactions has greatly advanced research in the fields of biology and medicine. They are not only valuable for labeling, tracking, and understanding biomolecules within living organisms, but also important for constructing advanced bioengineering and drug delivery systems. As the systems studied are increasingly complex, the simultaneous use of multiple bioorthogonal reactions is equally desirable. In this review, we take a look at the different bioorthogonal reactions that have recently been developed, the methods of cellular incorporation and the strategies to create orthogonality within the bioorthogonal landscape.
生物正交反应的出现极大地推动了生物学和医学领域的研究。它们不仅对标记、跟踪和了解生物体内的生物分子有价值，而且对构建先进的生物工程和药物传递系统也很重要。随着研究的系统变得越来越复杂，同时使用多种生物正交反应也是人们所期望的。在这篇综述中，我们研究了最近开发的不同生物正交反应、细胞掺入方法以及在生物正交环境中创造正交性的策略。
