School of Life Science, Taizhou University, 1139 Shifu Avenue, Taizhou 318000, China.
Jiangsu Yanghe Distillery Co., Suqian 223800, China.
Food Chem. 2021 Mar 1;339:128159. doi: 10.1016/j.foodchem.2020.128159. Epub 2020 Sep 25.
During production in Chinese baijiu fermentation process, huge amounts of the by-product vinasse are generated and generally utilized as low-value animal feed. We applied alkaline extraction in combination with ultrasonication to recover vinasse proteins, which were then hydrolyzed by complex protease Corolase PP for 8 h to obtain peptide fractions (VPH-1, -2, -3) displaying high DPPH radical scavenging activity. VPH-3 (<3 kDa) separated by ultrafiltration had EC values lower than those of VPH-1 and -2 for reactive oxygen species (ROS) and reactive nitrogen species (RNS) radicals, and significantly inhibited production of NO and pro-inflammatory cytokines in LPS-stimulated RAW264.7 macrophage cells. Active peptides and their amino acid sequences were identified by LC-MS/MS analysis, and five synthesized peptides (particularly KLPDHPKLPK and VDVPVKVPYS) displayed strong anti-inflammatory activity at concentration 0.25 mg/mL. These findings will be useful in future commercial development of baijiu vinasse, including application as a new source of bioactive peptides.
在白酒发酵过程中，会产生大量的副产物酒糟，通常用作低价值的动物饲料。我们应用碱性提取结合超声波处理来回收酒糟蛋白，然后用复合蛋白酶 Corolase PP 水解 8 小时，得到具有高 DPPH 自由基清除活性的肽段（VPH-1、-2、-3）。超滤分离得到的 VPH-3（<3 kDa）对活性氧（ROS）和活性氮（RNS）自由基的 EC 值低于 VPH-1 和 -2，并且显著抑制 LPS 刺激的 RAW264.7 巨噬细胞中 NO 和促炎细胞因子的产生。通过 LC-MS/MS 分析鉴定了活性肽及其氨基酸序列，五种合成肽（特别是 KLPDHPKLPK 和 VDVPVKVPYS）在 0.25 mg/mL 浓度下表现出很强的抗炎活性。这些发现将有助于未来白酒酒糟的商业开发，包括作为生物活性肽的新来源的应用。
