Widdershoven J, van Munster P, De Abreu R, Bosman H, van Lith T, van der Putten-van Meyel M, Motohara K, Matsuda I
Laboratory of the Department of Pediatrics and Surgery, Nijmegen University Hospital, The Netherlands.
Clin Chem. 1987 Nov;33(11):2074-8.
PIVKA-II (Protein Induced by Vitamin K Absence) is abnormal des-carboxylated prothrombin, which is present in vitamin K deficiency or in patients using warfarin. With a sensitive method for PIVKA-II, biochemical vitamin K deficiency can be established before clinical symptoms occur. We give an overview of methods used to detect PIVKA-II, and four selected methods are inter-compared: (a) measuring total factor II including PIVKA-II by using Echis carinatus snake venom as an activator of prothrombin; (b) measuring PIVKA-II by using snake venom as an activator of factor II after adsorption of functional factor II onto barium sulfate; (c) electrophoresis-immunofixation method; and (d) enzyme immunoassay. We found d to be the most sensitive and reliable method for PIVKA-II.
异常凝血酶原（PIVKA-II，维生素K缺乏诱导蛋白）是一种异常的脱羧凝血酶原，存在于维生素K缺乏的情况或使用华法林的患者体内。采用一种检测PIVKA-II的灵敏方法，可在临床症状出现之前确定生化性维生素K缺乏。我们综述了用于检测PIVKA-II的方法，并对四种选定的方法进行了相互比较：（a）使用锯鳞蝰蛇毒作为凝血酶原激活剂来测量包括PIVKA-II在内的总凝血因子II；（b）在功能性凝血因子II吸附到硫酸钡上之后，使用蛇毒作为凝血因子II的激活剂来测量PIVKA-II；（c）电泳免疫固定法；以及（d）酶免疫测定法。我们发现酶免疫测定法是检测PIVKA-II最灵敏且可靠的方法。
