COVID-19患者泪液中的抗SARS-CoV-2 IgA反应
Anti-SARS-Cov-2 IgA Response in Tears of COVID-19 Patients.
作者信息
Caselli Elisabetta, Soffritti Irene, Lamberti Giuseppe, D'Accolti Maria, Franco Filippo, Demaria Davide, Contoli Marco, Passaro Angela, Contini Carlo, Perri Paolo
机构信息
Department of Chemical and Pharmaceutical Sciences and LTTA, Section of Microbiology, University of Ferrara, 44121 Ferrara, Italy.
Department of Biomedical and Surgical-Specialized Sciences, Ophthalmology Unit, University of Ferrara, 44121 Ferrara, Italy.
出版信息
Biology (Basel). 2020 Nov 3;9(11):374. doi: 10.3390/biology9110374.
The pandemic virus SARS-CoV-2 has been reported to be able to enter the body via the eye conjunctiva, but the presence of antiviral response in the eye remains poorly known. Our study was thus aimed to analyze the presence of secretory mucosal anti-SARS-CoV-2 type A immunoglobulins (IgA) in the conjunctival fluid of COVID-19 patients. The tears of 28 COVID-19 patients and 20 uninfected controls were collected by the Schirmer test and analyzed by a specific ELISA assay detecting anti-spike (S1) virus protein IgA. The results showed that 35.7% of COVID-19 subjects have specific antiviral IgA at the ocular level, persisting till 48 days post disease onset. Most of the IgA positive subjects presented mild symptoms. The collected data indicate a prolonged persistence of anti-SARS-CoV-2 IgA at the eye level and suggest that IgA detection may be extremely helpful in clarifying virus pathology and epidemiology.
据报道，大流行病毒SARS-CoV-2能够通过眼结膜进入人体，但眼睛中抗病毒反应的存在情况仍知之甚少。因此，我们的研究旨在分析COVID-19患者结膜液中分泌型粘膜抗SARS-CoV-2 A型免疫球蛋白（IgA）的存在情况。通过Schirmer试验收集了28例COVID-19患者和20例未感染对照的眼泪，并通过检测抗刺突（S1）病毒蛋白IgA的特异性ELISA测定法进行分析。结果显示，35.7%的COVID-19受试者在眼部存在特异性抗病毒IgA，这种情况一直持续到疾病发作后48天。大多数IgA阳性受试者症状较轻。收集到的数据表明，抗SARS-CoV-2 IgA在眼部持续存在的时间较长，这表明IgA检测可能对阐明病毒病理学和流行病学非常有帮助。
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