Department of Orthopedic Trauma, The Affiliated Hospital of Chengde Medical College, China.
Department of Hand and Foot Surgery, The Affiliated Hospital of Chengde Medical College, China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820943217. doi: 10.1177/1533033820943217.
Circular RNAs are a recently discovered subclass of endogenous noncoding RNAs that have been confirmed to play an important role in various pathophysiological processes. However, the underlying function of circular RNAs in osteosarcoma still remains unclear. We aimed to comprehend the function of circ_0032462 in osteosarcoma, as it has been predicted to be highly expressed in osteosarcoma cells. Using real-time polymerase chain reaction, we verified the elevated expression of circ_0032462 in osteosarcoma cells than normal cells. Functional validation experiments revealed that circ_0032462 overexpression promoted proliferation, migration, and invasion in osteosarcoma cells, whereas circ_0032462 silencing was observed to inhibit cancer cell progression (proliferation, migration, and invasion). Furthermore, we found that circ_0032462 upregulated the messenger RNA and protein expression level of kinesin family member 3B. In addition, kinesin family member 3B inhibition was found to inhibit circ_0032462-induced enhanced osteosarcoma cell progression. circ_0032462 overexpression was observed to reverse circ_0032462 silencing-induced inhibitory effect on osteosarcoma cell progression. Overall, our research revealed the function of circ_0032462 in osteosarcoma progression, which might serve as a novel chemotherapeutic target for osteosarcoma.
环状 RNA 是最近发现的一类内源性非编码 RNA，已被证实在多种病理生理过程中发挥重要作用。然而，环状 RNA 在骨肉瘤中的潜在功能仍不清楚。我们旨在了解 circ_0032462 在骨肉瘤中的功能，因为它被预测在骨肉瘤细胞中高度表达。通过实时聚合酶链反应，我们验证了 circ_0032462 在骨肉瘤细胞中的表达高于正常细胞。功能验证实验表明，circ_0032462 的过表达促进了骨肉瘤细胞的增殖、迁移和侵袭，而 circ_0032462 的沉默被观察到抑制了癌细胞的进展（增殖、迁移和侵袭）。此外，我们发现 circ_0032462 上调了驱动蛋白家族成员 3B 的信使 RNA 和蛋白表达水平。此外，抑制驱动蛋白家族成员 3B 被发现抑制了 circ_0032462 诱导的骨肉瘤细胞进展增强。circ_0032462 的过表达观察到逆转了 circ_0032462 沉默对骨肉瘤细胞进展的抑制作用。总的来说，我们的研究揭示了 circ_0032462 在骨肉瘤进展中的作用，它可能成为骨肉瘤的一种新的化疗靶点。
