Orthopedic Center, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou City, 570208, Hainan, China.
J Orthop Surg Res. 2022 Sep 24;17(1):428. doi: 10.1186/s13018-022-03297-w.
Circular RNAs are involved in various cellular processes of bone diseases by acting as miRNA sponges to regulate gene expression levels, including osteosarcoma (OS). This research concentrated on the molecular mechanism of circ_0051079 in OS progression.
Reverse transcription-quantitative polymerase chain reaction assay was used for expression detection of circ_0051079, microRNA-1286 (miR-1286), and musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB). Cell Counting Kit-8 assay and Edu assay were used for cell proliferation analysis. Cell apoptosis was evaluated using flow cytometry. Western blot was performed to measure protein levels. Migration and invasion were assessed via transwell assay. Interaction of circ_0051079/miR-1286 or miR-1286/MAFB was explored through a dual-luciferase reporter assay. In vivo research was carried out via tumor xenograft assay and immunohistochemistry staining.
Circ_0051079 expression was upregulated in OS. Downregulation of circ_0051079 reduced OS cell proliferation, migration, invasion, and accelerated apoptosis. Circ_0051079 interacted with miR-1286, and the tumor-inhibitory function of si-circ_0051079 was abolished by miR-1286 inhibition in OS cells. MAFB served as a target for miR-1286. OS cell progression was suppressed by miR-1286 overexpression via downregulating MAFB. Circ_0051079/miR-1286 resulted in expression change of MAFB in OS cells. Silencing circ_0051079 inhibited tumor growth in vivo via regulating the miR-1286/MAFB axis.
The collective results elucidated that circ_0051079 contributed to OS progression via miR-1286-mediated upregulation of MAFB, confirming the interaction of circ_0051079/miR-1286/MAFB axis in OS.
环状 RNA 通过作为 miRNA 海绵来调节基因表达水平，参与骨疾病的各种细胞过程，包括骨肉瘤（OS）。本研究集中于 circ_0051079 在 OS 进展中的分子机制。
使用逆转录定量聚合酶链反应（RT-qPCR）检测 circ_0051079、microRNA-1286（miR-1286）和肌动蛋白-aponeurotic 纤维肉瘤癌基因同源物 B（MAFB）的表达。细胞计数试剂盒-8（CCK-8）和 Edu 检测用于细胞增殖分析。通过流式细胞术评估细胞凋亡。使用 Western blot 测定蛋白水平。通过 Transwell 测定评估迁移和侵袭。通过双荧光素酶报告基因检测探讨 circ_0051079/miR-1286 或 miR-1286/MAFB 的相互作用。通过肿瘤异种移植和免疫组化染色进行体内研究。
circ_0051079 在 OS 中表达上调。circ_0051079 下调可降低 OS 细胞增殖、迁移、侵袭并加速细胞凋亡。circ_0051079 与 miR-1286 相互作用，在 OS 细胞中，circ_0051079 的 siRNA 抑制作用可被 miR-1286 抑制所消除。MAFB 是 miR-1286 的靶标。通过下调 MAFB，miR-1286 过表达可抑制 OS 细胞的进展。circ_0051079/miR-1286 导致 OS 细胞中 MAFB 的表达变化。沉默 circ_0051079 通过调节 miR-1286/MAFB 轴抑制体内肿瘤生长。
研究结果表明，circ_0051079 通过 miR-1286 介导的 MAFB 上调促进 OS 进展，证实了 circ_0051079/miR-1286/MAFB 轴在 OS 中的相互作用。
