长链非编码RNA UCA1通过靶向微小RNA-193a-3p影响肝癌细胞的增殖、迁移、侵袭和凋亡。
LncRNA UCA1 Affects the Cell Proliferation, Migration, Invasion and Apoptosis of Hepatic Carcinoma Cells by Targeting MicroRNA-193a-3p.
作者信息
Wang Hong-Zhen, Liu Li, Xu Yan, Zhang Guang-Ye, Wang Yan-Yan
机构信息
Department of Oncology, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People's Republic of China.
Department of ENT, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People's Republic of China.
出版信息
Cancer Manag Res. 2020 Oct 30;12:10897-10907. doi: 10.2147/CMAR.S270396. eCollection 2020.
OBJECTIVE/BACKGROUND: Hepatic carcinoma (HCC) is the fourth lethal cancer in the world, but its relationship with lncRNA urothelial cancer-associated 1 (UCA1)/microRNA-193a-3p axis remains unclear, so this study would explore the relationship.
METHODS
A real-time polymerase chain reaction (RT-PCR) assay was carried out to quantify lncRNA UCA1 and microRNA-193a-3p in HCC tissues and cells, and relevant overexpression or inhibition vectors were constructed to analyze the influences of lncRNA UCA1 and microRNA-193a-3p on HCC cells. A Transwell assay was used to measure invasion and migration of HCC cells, and a Western blot assay to quantify protein biomarkers of apoptosis, invasion, and migration, a MTT assay to determine cell viability, a flow cytometry to detect cell cycle, and a dual-luciferase reporter gene assay to analyze the correlation between lncRNA UCA1 and microRNA-193a-3p.
RESULTS
LncRNA UCA1 was increased in HCC, while microRNA-193a-3p was decreased. Down-regulated lncRNA UCA1 could up-regulate microRNA-193a-3p, and down-regulated lncRNA UCA1 or up-regulated microRNA-193a-3p would strengthen cell apoptosis and weaken cell migration, invasion, and proliferation. Furthermore, lncRNA UCA1 could negatively regulate microRNA-193a-3p by binding to it.
CONCLUSION
LncRNA UCA1 promotes malignant hyperproliferation of HCC cells by repressing microRNA-193a-3p.
目的/背景：肝癌（HCC）是全球第四大致命性癌症，但其与长链非编码RNA尿路上皮癌相关1（UCA1）/微小RNA-193a-3p轴的关系仍不清楚，因此本研究将探索这种关系。
方法
采用实时聚合酶链反应（RT-PCR）检测法对肝癌组织和细胞中的长链非编码RNA UCA1和微小RNA-193a-3p进行定量，并构建相关的过表达或抑制载体，以分析长链非编码RNA UCA1和微小RNA-193a-3p对肝癌细胞的影响。采用Transwell检测法测量肝癌细胞的侵袭和迁移能力，采用蛋白质免疫印迹检测法对凋亡、侵袭和迁移的蛋白质生物标志物进行定量，采用MTT检测法测定细胞活力，采用流式细胞术检测细胞周期，并采用双荧光素酶报告基因检测法分析长链非编码RNA UCA1与微小RNA-193a-3p之间的相关性。
结果
肝癌中长链非编码RNA UCA1升高，而微小RNA-193a-3p降低。下调长链非编码RNA UCA1可上调微小RNA-193a-3p，而下调长链非编码RNA UCA1或上调微小RNA-193a-3p会增强细胞凋亡并减弱细胞迁移、侵袭和增殖。此外，长链非编码RNA UCA1可通过与微小RNA-193a-3p结合对其进行负调控。
结论
长链非编码RNA UCA1通过抑制微小RNA-193a-3p促进肝癌细胞的恶性增殖。
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