• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA UCA1通过靶向微小RNA-193a-3p影响肝癌细胞的增殖、迁移、侵袭和凋亡。

LncRNA UCA1 Affects the Cell Proliferation, Migration, Invasion and Apoptosis of Hepatic Carcinoma Cells by Targeting MicroRNA-193a-3p.

作者信息

Wang Hong-Zhen, Liu Li, Xu Yan, Zhang Guang-Ye, Wang Yan-Yan

机构信息

Department of Oncology, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People's Republic of China.

Department of ENT, Rizhao City Hospital of Traditional Chinese Medicine, Rizhao City, Shandong Province 276800, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Oct 30;12:10897-10907. doi: 10.2147/CMAR.S270396. eCollection 2020.

DOI:10.2147/CMAR.S270396
PMID:33154669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7608488/
Abstract

OBJECTIVE/BACKGROUND: Hepatic carcinoma (HCC) is the fourth lethal cancer in the world, but its relationship with lncRNA urothelial cancer-associated 1 (UCA1)/microRNA-193a-3p axis remains unclear, so this study would explore the relationship.

METHODS

A real-time polymerase chain reaction (RT-PCR) assay was carried out to quantify lncRNA UCA1 and microRNA-193a-3p in HCC tissues and cells, and relevant overexpression or inhibition vectors were constructed to analyze the influences of lncRNA UCA1 and microRNA-193a-3p on HCC cells. A Transwell assay was used to measure invasion and migration of HCC cells, and a Western blot assay to quantify protein biomarkers of apoptosis, invasion, and migration, a MTT assay to determine cell viability, a flow cytometry to detect cell cycle, and a dual-luciferase reporter gene assay to analyze the correlation between lncRNA UCA1 and microRNA-193a-3p.

RESULTS

LncRNA UCA1 was increased in HCC, while microRNA-193a-3p was decreased. Down-regulated lncRNA UCA1 could up-regulate microRNA-193a-3p, and down-regulated lncRNA UCA1 or up-regulated microRNA-193a-3p would strengthen cell apoptosis and weaken cell migration, invasion, and proliferation. Furthermore, lncRNA UCA1 could negatively regulate microRNA-193a-3p by binding to it.

CONCLUSION

LncRNA UCA1 promotes malignant hyperproliferation of HCC cells by repressing microRNA-193a-3p.

摘要

目的/背景：肝癌（HCC）是全球第四大致命性癌症，但其与长链非编码RNA尿路上皮癌相关1（UCA1）/微小RNA-193a-3p轴的关系仍不清楚，因此本研究将探索这种关系。

方法

采用实时聚合酶链反应（RT-PCR）检测法对肝癌组织和细胞中的长链非编码RNA UCA1和微小RNA-193a-3p进行定量，并构建相关的过表达或抑制载体，以分析长链非编码RNA UCA1和微小RNA-193a-3p对肝癌细胞的影响。采用Transwell检测法测量肝癌细胞的侵袭和迁移能力，采用蛋白质免疫印迹检测法对凋亡、侵袭和迁移的蛋白质生物标志物进行定量，采用MTT检测法测定细胞活力，采用流式细胞术检测细胞周期，并采用双荧光素酶报告基因检测法分析长链非编码RNA UCA1与微小RNA-193a-3p之间的相关性。

结果

肝癌中长链非编码RNA UCA1升高，而微小RNA-193a-3p降低。下调长链非编码RNA UCA1可上调微小RNA-193a-3p，而下调长链非编码RNA UCA1或上调微小RNA-193a-3p会增强细胞凋亡并减弱细胞迁移、侵袭和增殖。此外，长链非编码RNA UCA1可通过与微小RNA-193a-3p结合对其进行负调控。

结论

长链非编码RNA UCA1通过抑制微小RNA-193a-3p促进肝癌细胞的恶性增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/4318fc9e68f7/CMAR-12-10897-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/da0831284985/CMAR-12-10897-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/b2c1638148c7/CMAR-12-10897-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/31bb6eb523a8/CMAR-12-10897-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/db2e7af5f8ea/CMAR-12-10897-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/8ebcbeefcb7a/CMAR-12-10897-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/4318fc9e68f7/CMAR-12-10897-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/da0831284985/CMAR-12-10897-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/b2c1638148c7/CMAR-12-10897-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/31bb6eb523a8/CMAR-12-10897-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/db2e7af5f8ea/CMAR-12-10897-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/8ebcbeefcb7a/CMAR-12-10897-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7592/7608488/4318fc9e68f7/CMAR-12-10897-g0006.jpg

相似文献

1
LncRNA UCA1 Affects the Cell Proliferation, Migration, Invasion and Apoptosis of Hepatic Carcinoma Cells by Targeting MicroRNA-193a-3p.长链非编码RNA UCA1通过靶向微小RNA-193a-3p影响肝癌细胞的增殖、迁移、侵袭和凋亡。
Cancer Manag Res. 2020 Oct 30;12:10897-10907. doi: 10.2147/CMAR.S270396. eCollection 2020.
2
Knockdown of lncRNA-UCA1 inhibits cell viability and migration of human glioma cells by miR-193a-mediated downregulation of CDK6.敲低 lncRNA-UCA1 通过 miR-193a 介导的 CDK6 下调抑制人神经胶质瘤细胞的活力和迁移。
J Cell Biochem. 2019 Sep;120(9):15157-15169. doi: 10.1002/jcb.28777. Epub 2019 May 20.
3
SATB1 3'-UTR and lncRNA-UCA1 competitively bind to miR-495-3p and together regulate the proliferation and invasion of gastric cancer.SATB1 3'-UTR 和 lncRNA-UCA1 竞争性结合 miR-495-3p，并共同调节胃癌的增殖和侵袭。
J Cell Biochem. 2019 Apr;120(4):6671-6682. doi: 10.1002/jcb.27963. Epub 2018 Oct 28.
4
Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma.长链非编码 RNA UCA1 通过靶向口腔鳞状细胞癌中的 miR-143-3p 促进细胞生长、迁移和侵袭。
Cancer Med. 2020 May;9(9):3115-3129. doi: 10.1002/cam4.2808. Epub 2020 Mar 4.
5
LncRNA modulates the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the axis.长链非编码RNA通过调控该轴来调节肝癌细胞的增殖、迁移和侵袭。
Transl Cancer Res. 2024 Jan 31;13(1):423-436. doi: 10.21037/tcr-23-2228. Epub 2024 Jan 29.
6
MicroRNA-193a-3p participates in the progression of rheumatoid arthritis by regulating proliferation and apoptosis of MH7A cells through targeting IGFBP5.微小 RNA-193a-3p 通过靶向 IGFBP5 调节 MH7A 细胞的增殖和凋亡参与类风湿关节炎的进展。
Eur Rev Med Pharmacol Sci. 2019 Jun;23(11):4850-4857. doi: 10.26355/eurrev_201906_18072.
7
DNM3OS Facilitates Ovarian Cancer Progression by Regulating miR-193a-3p/MAP3K3 Axis.DNM3OS 通过调控 miR-193a-3p/MAP3K3 轴促进卵巢癌进展。
Yonsei Med J. 2021 Jun;62(6):535-544. doi: 10.3349/ymj.2021.62.6.535.
8
The LncRNA H19/miR-193a-3p axis modifies the radio-resistance and chemotherapeutic tolerance of hepatocellular carcinoma cells by targeting PSEN1.LncRNA H19/miR-193a-3p 轴通过靶向 PSEN1 修饰肝癌细胞的放射抵抗和化疗耐受性。
J Cell Biochem. 2018 Nov;119(10):8325-8335. doi: 10.1002/jcb.26883. Epub 2018 Jul 3.
9
Down-regulation of lncRNA UCA1 enhances radiosensitivity in prostate cancer by suppressing EIF4G1 expression via sponging miR-331-3p.lncRNA UCA1的下调通过海绵化miR-331-3p抑制EIF4G1表达来增强前列腺癌的放射敏感性。
Cancer Cell Int. 2020 Sep 11;20:449. doi: 10.1186/s12935-020-01538-8. eCollection 2020.
10
Long non-coding RNA UCA1 enhances cervical cancer cell proliferation and invasion by regulating microRNA-299-3p expression.长链非编码RNA UCA1通过调控微小RNA-299-3p的表达促进宫颈癌细胞的增殖和侵袭。
Oncol Lett. 2021 Nov;22(5):772. doi: 10.3892/ol.2021.13033. Epub 2021 Sep 9.

引用本文的文献

1
A New Understanding of Long Non-Coding RNA in Hepatocellular Carcinoma-From mA Modification to Blood Biomarkers.长链非编码 RNA 在肝细胞癌中的新认识——从 mA 修饰到血液生物标志物。
Cells. 2023 Sep 14;12(18):2272. doi: 10.3390/cells12182272.
2
Emerging role of long noncoding RNAs in recurrent hepatocellular carcinoma.长链非编码RNA在复发性肝细胞癌中的新作用
World J Clin Cases. 2021 Nov 16;9(32):9699-9710. doi: 10.12998/wjcc.v9.i32.9699.

本文引用的文献

1
Long noncoding RNA ZFAS1 promotes hepatocellular carcinoma proliferation by epigenetically repressing miR-193a-3p.长链非编码 RNA ZFAS1 通过表观遗传抑制 miR-193a-3p 促进肝癌增殖。
Eur Rev Med Pharmacol Sci. 2019 Nov;23(22):9840-9847. doi: 10.26355/eurrev_201911_19547.
2
Tumor Cell Biodiversity Drives Microenvironmental Reprogramming in Liver Cancer.肿瘤细胞多样性驱动肝癌微环境重编程。
Cancer Cell. 2019 Oct 14;36(4):418-430.e6. doi: 10.1016/j.ccell.2019.08.007. Epub 2019 Oct 3.
3
Obesity and liver cancer.肥胖与肝癌。
Ann Hepatol. 2019 Nov-Dec;18(6):810-815. doi: 10.1016/j.aohep.2019.07.004. Epub 2019 Aug 20.
4
MiRNA-124 inhibits the proliferation, migration and invasion of cancer cell in hepatocellular carcinoma by downregulating lncRNA-UCA1.微小RNA-124通过下调长链非编码RNA-UCA1抑制肝癌癌细胞的增殖、迁移和侵袭。
Onco Targets Ther. 2019 Jun 12;12:4509-4516. doi: 10.2147/OTT.S205169. eCollection 2019.
5
The changing epidemiology of primary liver cancer.原发性肝癌不断变化的流行病学。
Curr Epidemiol Rep. 2019 Jun;6(2):104-111. doi: 10.1007/s40471-019-00188-3. Epub 2019 May 3.
6
Statin use and risk of liver cancer: Evidence from two population-based studies.他汀类药物的使用与肝癌风险：来自两项基于人群的研究证据。
Int J Cancer. 2020 Mar 1;146(5):1250-1260. doi: 10.1002/ijc.32426. Epub 2019 Jun 4.
7
lncRNA UCA1-Mediated Cdc42 Signaling Promotes Oncolytic Vaccinia Virus Cell-to-Cell Spread in Ovarian Cancer.长链非编码RNA UCA1介导的Cdc42信号通路促进溶瘤痘苗病毒在卵巢癌中的细胞间传播
Mol Ther Oncolytics. 2019 Mar 26;13:35-48. doi: 10.1016/j.omto.2019.03.003. eCollection 2019 Jun 28.
8
Long noncoding RNA SNHG14 promotes breast cancer cell proliferation and invasion via sponging miR-193a-3p.长链非编码 RNA SNHG14 通过海绵吸附 miR-193a-3p 促进乳腺癌细胞增殖和侵袭。
Eur Rev Med Pharmacol Sci. 2019 Mar;23(6):2461-2468. doi: 10.26355/eurrev_201903_17393.
9
Circular RNA circHIPK3 promotes cell proliferation and invasion of prostate cancer by sponging miR-193a-3p and regulating MCL1 expression.环状RNA circHIPK3通过吸附miR-193a-3p并调节MCL1表达促进前列腺癌细胞增殖和侵袭。
Cancer Manag Res. 2019 Feb 12;11:1415-1423. doi: 10.2147/CMAR.S190669. eCollection 2019.
10
Hepatocellular carcinoma in a rapidly growing community: Epidemiology, clinico-pathology and predictors of extrahepatic metastasis.快速发展社区中的肝细胞癌：肝外转移的流行病学、临床病理学及预测因素
Arab J Gastroenterol. 2019 Mar;20(1):38-43. doi: 10.1016/j.ajg.2019.01.006. Epub 2019 Mar 8.