Zhao Baolei, Lu Yanmin, Cao Xuefeng, Zhu Wentao, Kong Lingqun, Ji Haibin, Zhang Fan, Lin Xutao, Guan Qinghai, Ou Kun, Zhang Xingyuan, Chen Qiangpu
Department of Hepatobiliary Surgery, Binzhou Medical University Hospital, Binzhou City, Shandong Province 256603, People's Republic of China.
Onco Targets Ther. 2019 Jun 12;12:4509-4516. doi: 10.2147/OTT.S205169. eCollection 2019.
It has been reported that miRNA-124 inhibits hepatocellular carcinoma (HCC) progression, while lncRNA-UCA1 promotes HCC. The aim of this study is to find whether miRNA-124, as a tumor suppressor in HCC can inhibit lncRNA-UCA1 in HCC cell. Tumor tissues and adjacent healthy tissues were obtained from 66 patients diagnosed with HCC in Binzhou Medical University Hospital from January 2011 to January 2013. Cell proliferation assay, in vitro cell migration and invasion assay were applied for the research. In the present study we found that miRNA-124 was downregulated, while lncRNA-UCA1 was upregulated in tumor tissues comparing to adjacent healthy tissues of HCC patients. Expression of miRNA-124 and lncRNA-UCA1 in tumor tissues were not affected by HBV or HCV infection. Analysis of followed-up data revealed that low miRNA-124 level and high lncRNA-UCA1 level were closely correlated with poor survival. Overexpression of miRNA-124 led to inhibited lncRNA-UCA1 expression in cells of HCC cell lines, while overexpression of lncRNA-UCA1 failed to significantly affect miRNA-124 expression. Expression levels of miRNA-124 and lncRNA-UCA1 were inversely and significantly correlated in tumor tissues but not in adjacent healthy tissues. Overexpression of miRNA-124 led to inhibited, while overexpression of lncRNA-UCA1 led to increased proliferation, migration and invasion rates of HCC cell lines. In addition, lncRNA-UCA1 overexpression attenuated the inhibitory effects of miRNA-124 overexpression on cancer cell proliferation, migration and invasion. Therefore, miRNA-124 may inhibit the proliferation, migration and invasion of cancer cell in hepatocellular carcinoma by downregulating lncRNA-UCA1.
据报道，miRNA - 124抑制肝细胞癌（HCC）进展，而lncRNA - UCA1促进HCC。本研究旨在探究作为HCC肿瘤抑制因子的miRNA - 124是否能在HCC细胞中抑制lncRNA - UCA1。2011年1月至2013年1月期间，从滨州医学院附属医院66例诊断为HCC的患者中获取肿瘤组织及相邻健康组织。采用细胞增殖试验、体外细胞迁移和侵袭试验进行研究。在本研究中，我们发现与HCC患者的相邻健康组织相比，肿瘤组织中miRNA - 124表达下调，而lncRNA - UCA1表达上调。肿瘤组织中miRNA - 124和lncRNA - UCA1的表达不受HBV或HCV感染影响。随访数据分析显示，低miRNA - 124水平和高lncRNA - UCA1水平与不良生存密切相关。miRNA - 124过表达导致HCC细胞系细胞中lncRNA - UCA1表达受到抑制，而lncRNA - UCA1过表达未能显著影响miRNA - 124表达。miRNA - 124和lncRNA - UCA1的表达水平在肿瘤组织中呈显著负相关，而在相邻健康组织中无此相关性。miRNA - 124过表达导致HCC细胞系细胞的增殖、迁移和侵袭率受到抑制，而lncRNA - UCA1过表达导致这些指标升高。此外，lncRNA - UCA1过表达减弱了miRNA - 124过表达对癌细胞增殖、迁移和侵袭的抑制作用。因此，miRNA - 124可能通过下调lncRNA - UCA1抑制肝细胞癌癌细胞的增殖、迁移和侵袭。
