鉴定微染色体维持蛋白 8 作为一个潜在的预后标志物及其对胃癌增殖和凋亡的影响。
Identification of mini-chromosome maintenance 8 as a potential prognostic marker and its effects on proliferation and apoptosis in gastric cancer.
机构信息
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
出版信息
J Cell Mol Med. 2020 Dec;24(24):14415-14425. doi: 10.1111/jcmm.16062. Epub 2020 Nov 6.
Mini-chromosome maintenance (MCM) proteins play important roles in initiating eukaryotic genome replication. The MCM family of proteins includes several members associated with the development and progression of certain cancers. We performed online data mining to assess the expression of MCMs in gastric cancer (GC) and the correlation between their expression and survival in patients with GC. Notably, MCM8 expression was undoubtedly up-regulated in GC, and higher expression correlated with shorter overall survival (OS) and progression-free survival (PFS) in patients with GC. However, the role of MCM8 in GC has not been previously explored. Our in vitro experiments revealed that MCM8 knockdown inhibited cell growth and metastasis. Moreover, MCM8 knockdown induced apoptosis. Mechanistically, the expression levels of Bax and cleaved caspase-3 were increased, whereas Bcl-2 expression decreased. Additionally, we demonstrated that MCM8 knockdown suppressed tumorigenesis in vivo. Overall, these results suggest that MCM8 plays a significant role in GC progression.
微小染色体维持蛋白（MCM）在启动真核生物基因组复制中发挥着重要作用。MCM 蛋白家族的几个成员与某些癌症的发生和进展有关。我们进行了在线数据挖掘，以评估 MCM 在胃癌（GC）中的表达情况及其与 GC 患者生存的相关性。值得注意的是，MCM8 在 GC 中表达明显上调，并且在 GC 患者中，高表达与总生存期（OS）和无进展生存期（PFS）更短相关。然而，MCM8 在 GC 中的作用尚未被探索。我们的体外实验表明，MCM8 敲低抑制了细胞生长和转移。此外，MCM8 敲低诱导了细胞凋亡。机制上，Bax 和 cleaved caspase-3 的表达水平增加，而 Bcl-2 的表达水平降低。此外，我们证明了 MCM8 敲低在体内抑制了肿瘤发生。总的来说，这些结果表明 MCM8 在 GC 进展中起着重要作用。
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