College of Food Science and Engineering, Nanjing University of Finance and Economics/Key Laboratory of Grains and Oils Quality Control and Processing/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China.
Food Funct. 2020 Nov 18;11(11):10161-10169. doi: 10.1039/d0fo02285c.
5-n-Alkylresorcinols (ARs) are abundant in wheat bran and potentially antioxidative, although the neuroprotective mechanism is not fully understood. The neuroprotective effect of wheat bran ARs on H2O2-induced neuronal cells and the relationship between neuroprotection and the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant-response element (ARE) pathway were investigated in this study. Seven homologs were identified in the purified ARs by high-performance liquid chromatography-tandem mass spectrometry. Pretreatment with 80 μg mL-1 ARs alleviated 23% HT22 cell death and the up-regulation of intracellular reactive oxygen species level and malondialdehyde under H2O2 stimulation. The neuroprotection effect was proved by the increase in the Nrf2 nuclear location and up-regulation of mRNA and protein expressions of heme oxygenase-1, NAD(P)H quinone dehydrogenase 1, glutamate-cysteine ligase catalytic subunit, and glutamate-cysteine ligase modifier subunit l. Wheat bran ARs displayed a neuroprotective function, possibly by promoting the endogenous antioxidant defense system. ARs may be regarded as a functional food ingredient for preventing neurodegenerative diseases in the future.
5- 正烷基间苯二酚（ARs）在麦麸中含量丰富，具有潜在的抗氧化作用，但其神经保护机制尚不完全清楚。本研究旨在探讨麦麸 ARs 对 H2O2 诱导的神经元细胞的神经保护作用及其与核因子红细胞 2 相关因子 2（Nrf2）/抗氧化反应元件（ARE）通路的关系。通过高效液相色谱-串联质谱法鉴定出纯化的 ARs 中有 7 种同系物。80μg mL-1ARs 预处理可减轻 23%HT22 细胞死亡，并在 H2O2 刺激下上调细胞内活性氧水平和丙二醛含量。通过增加 Nrf2 核定位以及血红素加氧酶-1、NAD（P）H 醌氧化还原酶 1、谷氨酰胺半胱氨酸连接酶催化亚基和谷氨酰胺半胱氨酸连接酶修饰亚基 l 的 mRNA 和蛋白表达，证明了这种神经保护作用。麦麸 ARs 具有神经保护功能，可能通过促进内源性抗氧化防御系统。ARs 将来可能被视为预防神经退行性疾病的功能性食品成分。
