Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.
Probiotics Antimicrob Proteins. 2021 Jun;13(3):847-861. doi: 10.1007/s12602-020-09714-y. Epub 2020 Nov 6.
The therapeutic application of bromelain is limited due to its sensitivity to operating conditions such as high acidity, gastric proteases in the stomach juice, chemicals, organic solvents and elevated temperature. We hypothesized that bromelain immobilized on probiotic bacterial spores would show enhanced therapeutic activity through possible synergistic or additive effects. In this study, the oedema inhibition potential of bromelain immobilized on probiotic Bacillus spores was compared to the free enzyme using the carrageenan paw oedema model with Wistar rats. In batch A rats (carrageenan-induced inflammation 30 min after receiving oral treatments), group 7 rats treated with a lower dose of spore-immobilized bromelain suspension showed the highest oedema inhibition, 89.20 ± 15.30%, while group 4 treated with a lower dose of free bromelain had oedema inhibition of 60.25 ± 13.00%. For batch B rats (carrageenan-induced inflammation after receiving oral treatment for three days), group 7 rats treated with a lower dose of spore-immobilized bromelain suspension showed higher inhibition percentage (81.94 ± 8.86) than group 4 treated with a lower dose of free bromelain (78.45 ± 4.46) after 24 h. Our results showed that used alone, the enzyme and the spores produced oedema inhibition and improved the motility of the rats. The spore-immobilized bromelain formulation performed approximately 0.9-fold better than the free bromelain and the free spores at the lower evaluated dose.
由于菠萝蛋白酶对操作条件（如高酸度、胃液中的胃蛋白酶、化学物质、有机溶剂和高温）敏感，因此其治疗应用受到限制。我们假设，固定在益生菌细菌孢子上的菠萝蛋白酶通过可能的协同或附加作用会显示出增强的治疗活性。在这项研究中，使用角叉菜胶足肿胀模型，用 Wistar 大鼠比较了固定在益生菌芽孢上的菠萝蛋白酶与游离酶的水肿抑制潜力。在批次 A 大鼠（在接受口服治疗 30 分钟后发生角叉菜胶诱导的炎症）中，用较低剂量的孢子固定化菠萝蛋白酶悬浮液治疗的第 7 组显示出最高的水肿抑制率，为 89.20±15.30%，而用较低剂量的游离菠萝蛋白酶治疗的第 4 组的水肿抑制率为 60.25±13.00%。对于批次 B 大鼠（在接受口服治疗三天后发生角叉菜胶诱导的炎症），用较低剂量的孢子固定化菠萝蛋白酶悬浮液治疗的第 7 组在 24 小时后显示出更高的抑制百分比（81.94±8.86），而用较低剂量的游离菠萝蛋白酶治疗的第 4 组为 78.45±4.46%。我们的结果表明，单独使用时，酶和孢子都能抑制水肿并改善大鼠的运动能力。与游离的菠萝蛋白酶和游离的孢子相比，固定化的菠萝蛋白酶制剂在较低的评估剂量下的效果大约提高了 0.9 倍。
