Archer Ann Catherine, Muthukumar S P, Halami Prakash M
Microbiology and Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
Animal House Facility, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
Int J Biol Macromol. 2015 Nov;81:530-7. doi: 10.1016/j.ijbiomac.2015.08.044. Epub 2015 Aug 24.
The aim of the present study was to evaluate the anti-inflammatory ability of novel indigenous probiotic Lactobacillus fermentum MCC 2759, L. fermentum MCC 2760 and Lactobacillus delbrueckii MCC 2775 in a carrageenan induced acute inflammatory paw edema model. Probiotic cultures were administered to male Wistar rats via oral route. Carrageenan at a concentration of 1% was injected into hind paw of rats 30min after oral gavage on the 8th day of treatment regimen. Paw thickness (mm), stair climbing activity and motility score were the parameters used to score the inflammatory response. L. fermentum MCC 2759, L. fermentum MCC 2760 and L. delbrueckii MCC 2775 showed significant reduction in paw thickness (P<0.05) showing percentage inhibition of 15.67%, 14.72% and 14.84%, respectively, 24h after carrageenan induction. Probiotic treatment also markedly alleviated the stair climbing and motility score. Histological analysis of tissue sections revealed reduction in cellular infiltration of probiotic and drug treatment groups. Adhesion to resected rat intestinal tissue also showed significant adherence capability (>40%) of the probiotic cultures used. Therefore, L. fermentum MCC 2759, L. fermentum MCC 2760 and L. delbrueckii MCC 2775 may be used as potent anti-inflammatory agents with probiotic health benefits.
本研究的目的是在角叉菜胶诱导的急性炎症性爪水肿模型中评估新型本土益生菌发酵乳杆菌MCC 2759、发酵乳杆菌MCC 2760和德氏乳杆菌MCC 2775的抗炎能力。通过口服途径将益生菌培养物给予雄性Wistar大鼠。在治疗方案的第8天，口服灌胃30分钟后，将浓度为1%的角叉菜胶注射到大鼠的后爪中。爪厚度（毫米）、爬楼梯活动和运动评分是用于评估炎症反应的参数。在角叉菜胶诱导后24小时，发酵乳杆菌MCC 2759、发酵乳杆菌MCC 2760和德氏乳杆菌MCC 2775的爪厚度显著降低（P<0.05），抑制率分别为15.67%、14.72%和14.84%。益生菌治疗还显著减轻了爬楼梯和运动评分。组织切片的组织学分析显示，益生菌和药物治疗组的细胞浸润减少。对切除的大鼠肠道组织的黏附也显示所用益生菌培养物具有显著的黏附能力（>40%）。因此，发酵乳杆菌MCC 2759、发酵乳杆菌MCC 2760和德氏乳杆菌MCC 2775可作为具有益生菌健康益处的强效抗炎剂。
