Komárková Martina, Chromý Jakub, Pokorná Eva, Soudek Petr, Máchová Pavlína
Forestry and Game Management Research Institute, Strnady, 25202 Jiloviste, Czech Republic.
The Czech Academy of Sciences, Institute of Experimental Botany, 16502 Prague, Czech Republic.
Plants (Basel). 2020 Nov 4;9(11):1485. doi: 10.3390/plants9111485.
(1) Background: (Aiton) Sm. is a fast-growing woody plant belonging to the family . Two poplar genotypes characterized by unique phenotypic traits (TP11 and TP20) were chosen to be characterized and tested for a physiological and transcriptomic response to Cd stress. (2) Methods: A comparative analysis of the effects of exposure to high cadmium (Cd) concentrations (10 µM and 100 µM) of TP11 and TP20 was performed. (3) Results: Neither of the tested Cd concentration negatively affected plant growth; however, the chlorophyll content significantly decreased. The potassium (K) content was higher in the shoots than in the roots. The magnesium concentrations were only slightly affected by Cd treatment. The zinc content in the shoots of TP20 was lower than that in the shoots of TP11. Cd accumulation was higher in the roots than in the shoots. After 10 days of exposure, 10 µM Cd resulted in comparable amounts of Cd in the roots and shoots of TP20. The most significant change in transcript amount was observed in endochitinase 2, 12-oxophytodienoate reductase 1 and phi classglutathione S-transferase. (4) Conclusions: Our study provided new insights for effective assessing the ability of different poplar genotypes to tolerate Cd stress and underlying Cd tolerance.
(1) 背景：(Aiton) Sm. 是一种生长迅速的木本植物，属于[此处原文缺失科名]科。选择了两种具有独特表型特征的杨树基因型（TP11和TP20），对其进行镉胁迫下的生理和转录组反应的表征与测试。(2) 方法：对TP11和TP20暴露于高镉（Cd）浓度（10 μM和100 μM）的影响进行了比较分析。(3) 结果：所测试的镉浓度均未对植物生长产生负面影响；然而，叶绿素含量显著下降。地上部分的钾（K）含量高于根部。镁浓度仅受到镉处理的轻微影响。TP20地上部分的锌含量低于TP11地上部分。镉在根部的积累高于地上部分。暴露10天后，10 μM镉导致TP20根部和地上部分的镉含量相当。转录量变化最显著的是内切几丁质酶2、12-氧代植物二烯酸还原酶1和phi类谷胱甘肽S-转移酶。(4) 结论：我们的研究为有效评估不同杨树基因型耐受镉胁迫的能力及潜在的镉耐受性提供了新的见解。
