State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
New Rural Research Institute in Lianyungang, Nanjing Agricultural University, Nanjing 210095, China.
Biomed Res Int. 2018 May 24;2018:6023457. doi: 10.1155/2018/6023457. eCollection 2018.
The GSTs is one of the most important multifunctional protein families which has been playing a crucial role in the different aspects of plant growth. This extensive study about GSTs may establish a solid foundation for the brief functional analysis of BraGSTs in future. In this study, a total of 75 genes were identified in . Phylogenetic analysis characterized them into eight different subclasses, while Tau and Phi subclasses were the most numerous. The exon-intron structure and the motif composition of BraGSTs were exhibited accordingly to their subclasses. Notably, we also investigated 15 tandem paralogous pairs of genes, which highlighted that all the pairs were purifying in nature as their synonymous values were lower than 1.00. Duplication analysis indicated that about 45.33% of genes mainly occurred through tandem duplication in . Predominately, the tandem cluster of genes in subclass Tau was greater than the other subclasses. Furthermore, among eight multiple hormonal treatments (ABA, GA, BR, ETH, IAA, IBA, NPA, and JA), most number of BraGSTs was activated by NPA, BR, and ABA treatments. This analysis has provided comprehensive information about GSTs family which may assist in elucidating their exact functions in .
谷胱甘肽硫转移酶（GSTs）是最重要的多功能蛋白家族之一，在植物生长的各个方面都起着至关重要的作用。对 GSTs 的广泛研究可能为今后简要分析 BraGSTs 的功能奠定坚实的基础。本研究在 中鉴定出了 75 个基因。系统发育分析将它们分为 8 个不同的亚科，其中 Tau 和 Phi 亚科数量最多。根据 BraGSTs 的亚科，展示了它们的exon-intron 结构和基序组成。值得注意的是，我们还研究了 15 对串联的基因对，这表明所有的基因对都是纯化的，因为它们的同义值都低于 1.00。复制分析表明，大约 45.33%的基因主要通过 中的串联复制发生。主要的是，Tau 亚科中的基因串联簇大于其他亚科。此外，在 8 种多种激素处理（ABA、GA、BR、ETH、IAA、IBA、NPA 和 JA）中，大多数 BraGSTs 被 NPA、BR 和 ABA 处理激活。这项分析提供了 GSTs 家族的全面信息，这可能有助于阐明它们在 中的确切功能。
