Department of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, Smoluchowskiego 25, 50-372, Wrocław, Poland.
Department of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, Smoluchowskiego 25, 50-372, Wrocław, Poland.
Environ Pollut. 2021 Jan 1;268(Pt B):115937. doi: 10.1016/j.envpol.2020.115937. Epub 2020 Oct 28.
The aim of this work was to prepare an innovative microelemental feed additive for laying hens, based on waste biomass from the agricultural sector (alfalfa and goldenrod after CO extraction in supercritical state). The process was optimized by Response Surface Methodology (RSM) and the most favourable enrichment conditions were selected for Cu(II), Mn(II) and Zn(II) ions: pH - 5, sorbate concentration of Cu(II), Mn(II), Zn(II) - 10.0 mg/L for alfalfa and 10.7 mg/L for goldenrod and biomass dose - 0.1 g/L. Physicochemical properties of biomass were studied and functional groups involved in the binding of Cu(II), Mn(II), Zn(II) ions were determined (mainly carboxylic and hydroxylic groups). An interesting and unique element of this work is the verification of the properties of prepared feed additives in conditions simulating the digestive tract of animals. The release of components in solutions simulating conditions in the intestine and stomach (pH 11 and pH 1) was tested (in vitro tests). The best desorption results were achieved at a strongly acidic pH which corresponds to the stomach environment: 9.80, 14.4% Cu(II), 69.0, 66.9% (Zn), 46.5, 31.9 Mn(II) for alfalfa and goldenrod, respectively. It was concluded that the biomass enriched with micronutrients in biosorption has the potential as a feed additive for sustainable agriculture.
这项工作的目的是基于农业部门（超临界 CO 提取后的紫花苜蓿和一枝黄花）的废生物质，制备一种用于蛋鸡的新型微量元素饲料添加剂。该过程通过响应面法（RSM）进行了优化，并选择了最有利于 Cu(II)、Mn(II)和 Zn(II)离子富集的条件：pH-5，Cu(II)、Mn(II)、Zn(II)的吸附剂浓度-紫花苜蓿和一枝黄花分别为 10.0mg/L 和 10.7mg/L，生物质剂量-0.1g/L。研究了生物质的物理化学性质，并确定了参与 Cu(II)、Mn(II)、Zn(II)离子结合的功能基团（主要是羧基和羟基）。这项工作的一个有趣和独特的元素是在模拟动物消化道条件下验证制备的饲料添加剂的性质。在模拟肠道和胃条件的溶液中（pH 11 和 pH 1）测试了成分的释放（体外测试）。在与胃环境相对应的强酸性 pH 条件下，达到了最佳的解吸效果：紫花苜蓿和一枝黄花的 Cu(II)分别为 9.80、14.4%，Zn(II)分别为 69.0、66.9%，Mn(II)分别为 46.5、31.9%。研究结论认为，在生物吸附中富含微量元素的生物质具有作为可持续农业饲料添加剂的潜力。
