Household & Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.
Forensic Sci Med Pathol. 2020 Dec;16(4):664-670. doi: 10.1007/s12024-020-00318-0. Epub 2020 Nov 6.
The ability to isolate and generate a DNA profile from human DNA recovered from tropical bed bugs (Cimex hemipterus) for identifying individuals can be useful for public health, forensic, and medical entomology. In this study, genomic DNA was recovered from both male and female bed bugs at every time interval tested (0, 1, 3, 5, 7, 14, 30, and 45 days post blood meal). The total DNA concentrations recovered from male bed bugs ranged from 12.93 to 65.97 ng/µL, while the total DNA concentrations from female bed bugs ranged from 8.93 to 44.53 ng/µL. However, based on the results from the BLAST search and PCR products, human DNA could be detected from female bed bugs at 0, 3, 5, 14, and 30 days post blood meal using the D18S51 marker. Concentrations of PCR products of the D18S51 locus from male bed bugs ranged from 4.20 to 35.50 ng/µL, whereas, for female bed bugs, concentrations ranged from 4.31 to 22.47 ng/µL. These were generally higher compared to the PCR products of the first hypervariable part (HVR1) marker. The results indicate the HVR1 locus was less sensitive than the D18S51 locus.
从热带臭虫（Cimex hemipterus）中回收的人类 DNA 进行 DNA 图谱分析以鉴定个体的能力对公共卫生、法医学和医学昆虫学很有用。在这项研究中，从雄性和雌性臭虫中在每个测试时间间隔（饱血后 0、1、3、5、7、14、30 和 45 天）回收基因组 DNA。从雄性臭虫中回收的总 DNA 浓度范围为 12.93 至 65.97ng/µL，而从雌性臭虫中回收的总 DNA 浓度范围为 8.93 至 44.53ng/µL。然而，根据 BLAST 搜索和 PCR 产物的结果，使用 D18S51 标记，可以从饱血后 0、3、5、14 和 30 天的雌性臭虫中检测到人类 DNA。来自雄性臭虫的 D18S51 基因座的 PCR 产物浓度范围为 4.20 至 35.50ng/µL，而雌性臭虫的浓度范围为 4.31 至 22.47ng/µL。这些浓度通常比第一高变区（HVR1）标记的 PCR 产物高。结果表明，HVR1 基因座的灵敏度低于 D18S51 基因座。
