Lim Li, Ab Majid Abdul Hafiz
Household and Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Sci Rep. 2021 Apr 19;11(1):8465. doi: 10.1038/s41598-021-87946-w.
With the development of new metagenomic techniques, the microbial community structure of common bed bugs, Cimex lectularius, is well-studied, while information regarding the constituents of the bacterial communities associated with tropical bed bugs, Cimex hemipterus, is lacking. In this study, the bacteria communities in the blood-fed and starved tropical bed bugs were analysed and characterized by amplifying the v3-v4 hypervariable region of the 16S rRNA gene region, followed by MiSeq Illumina sequencing. Across all samples, Proteobacteria made up more than 99% of the microbial community. An alpha-proteobacterium Wolbachia and gamma-proteobacterium, including Dickeya chrysanthemi and Pseudomonas, were the dominant OTUs at the genus level. Although the dominant OTUs of bacterial communities of blood-fed and starved bed bugs were the same, bacterial genera present in lower numbers were varied. The bacteria load in starved bed bugs was also higher than blood-fed bed bugs.
随着新的宏基因组技术的发展，温带臭虫（Cimex lectularius）的微生物群落结构已得到充分研究，而关于热带臭虫（Cimex hemipterus）相关细菌群落组成的信息却很缺乏。在本研究中，通过扩增16S rRNA基因区域的v3 - v4高变区，随后进行MiSeq Illumina测序，对吸血和饥饿状态下的热带臭虫体内细菌群落进行了分析和表征。在所有样本中，变形菌门占微生物群落的99%以上。α - 变形菌沃尔巴克氏体属以及γ - 变形菌属（包括菊欧文氏菌和假单胞菌属）是属水平上的优势操作分类单元。虽然吸血和饥饿臭虫细菌群落的优势操作分类单元相同，但数量较少的细菌属有所不同。饥饿臭虫体内的细菌载量也高于吸血臭虫。
