防腐剂筛选及毒性研究用纤维素纳米纤维的灭菌评估。
Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies.
机构信息
Innovation Promotion Division, Oji Holdings Corporation, Tokyo, Japan.
Research Institute of Science and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
出版信息
J Occup Health. 2020 Jan;62(1):e12176. doi: 10.1002/1348-9585.12176.
OBJECTIVES
The aim of this study is to establish a sterilization method for cellulose nanofibers (CNFs) dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs, and to provide useful information for sample preparation in future toxicity study of CNFs.
METHODS
Various preservatives were added to the phosphorylated CNF dispersions, endotoxin level and the numbers of bacteria and fungi in the CNF dispersion were analyzed. The pH values and viscosity of sterilized CNF dispersions were compared with those of control and autoclaved CNF dispersions.
RESULTS
Phosphorylated CNF dispersions at a concentration of 2.0 mg/mL or lower and the addition of 10 µg/mL benzalkonium chloride alone or 250 µg/mL methyl parahydroxybenzoate and 250 µg/mL propyl parahydroxybenzoate in combination can sterilize CNF dispersions without changing the physical and chemical properties of CNFs.
CONCLUSIONS
We developed sterilization method for CNF dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs. This sterilization method for CNFs dispersions can be applied to the safety assessment of CNF with different physicochemical properties in the future.
目的
本研究旨在建立一种使用不同亲水性防腐剂对纤维素纳米纤维（CNF）分散体进行消毒的方法，且不影响 CNF 的物理化学性质，为未来 CNF 毒性研究的样品制备提供有用信息。
方法
向磷酸化 CNF 分散体中添加各种防腐剂，分析 CNF 分散体中的内毒素水平和细菌真菌数量。比较消毒后 CNF 分散体的 pH 值和粘度与对照和高压灭菌 CNF 分散体的差异。
结果
浓度为 2.0mg/mL 或更低的磷酸化 CNF 分散体，以及单独添加 10μg/mL 苯扎氯铵或 250μg/mL 对羟基苯甲酸甲酯和 250μg/mL 对羟基苯甲酸丙酯组合，可以对 CNF 分散体进行消毒，而不改变 CNF 的物理化学性质。
结论
我们开发了一种使用不同亲水性防腐剂对 CNF 分散体进行消毒的方法，且不影响 CNF 的物理化学性质。这种 CNF 分散体的消毒方法可应用于未来对不同物理化学性质的 CNF 进行安全性评估。
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